PDF(Pubmed)
Abstract：
Diabetes mellitus (DM) is one of the leading worldwide public health problems. It is characterized by hyperglycemia which induces oxidative stress and inflammation, both involved in the pathogenesis of diabetes. We previously showed that Boswellia dalzielii (BD) and Hibiscus sabdariffa (HS) extracts reduced hyperglycemia and hyperlipidemia in alloxan-induced diabetic rats. In the present study, we evaluated the antioxidant and anti-inflammatory activities of both plants in alloxan-induced diabetic rats. Two sets of experiments were conducted in male Wistar rats subjected to a single intraperitoneal injection of alloxan monohydrate (150 mg/kg, b. w.). Then, diabetic rats were daily administered with either BD (1st set of experiments) or HS (2nd set of experiments) at 100, 200, and 400 mg/kg orally for 21 consecutive days. Glibenclamide (10 mg/kg) was also administered as a reference drug. At the end of the study, the animals were anesthetized, and blood samples were collected from each animal. Then, oxidative stress and inflammatory biomarkers in the serum were determined. We found that treatment with BD and HS significantly reduced malondialdehyde (MDA) and enhanced the levels of reduced glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT). These extracts also significantly decreased the inflammatory markers tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β). From the results obtained, it can therefore be concluded that BD and HS have the potential to being developed as natural sources of antioxidant and anti-inflammatory agents that can be used for the prevention or treatment of DM.
摘要：
糖尿病（DM）是全球主要的公共卫生问题之一。它的特点是高血糖，引起氧化应激和炎症，两者都参与了糖尿病的发病机制。我们先前表明，Boswelliadalzielii（BD）和Hibiscussabdariffa（HS）提取物可降低四氧嘧啶诱导的糖尿病大鼠的高血糖和高脂血症。在本研究中,我们评估了两种植物在四氧嘧啶诱导的糖尿病大鼠中的抗氧化和抗炎活性。在雄性Wistar大鼠中进行了两组实验，该大鼠接受了一次腹膜内注射四氧嘧啶一水合物（150mg/kg，b.w.)。然后，糖尿病大鼠每天口服100,200和400mg/kg的BD(第一组实验)或HS(第二组实验),连续21天.格列本脲(10mg/kg)也作为参比药物给药。在研究结束时,动物被麻醉，并从每只动物收集血液样本。然后，确定血清中的氧化应激和炎症生物标志物。我们发现，用BD和HS治疗显着降低丙二醛（MDA）和提高还原型谷胱甘肽（GSH）的水平，超氧化物歧化酶（SOD），和过氧化氢酶(CAT)。这些提取物还显着降低了炎症标志物肿瘤坏死因子-α（TNF-α），白细胞介素-6（IL-6），白细胞介素-1β(IL-1β)。从获得的结果来看，因此,可以得出结论,BD和HS具有被开发为可用于预防或治疗DM的抗氧化剂和抗炎剂的天然来源的潜力。
