背景:皮瓣移植是外科手术中广泛使用的塑料修复技术,旨在解决各种伤口和疾病导致的皮肤缺陷。然而,由于皮瓣手术后血液供应不足,缺血再灌注损伤的发生,和过度的无菌炎症反应,皮瓣经常发生并发症(例如,部分或完全缺血性坏死)。这些并发症对伤口愈合和修复有不良影响。β-石竹烯(BCP)是广泛存在于植物中的双环倍半萜。它减轻氧化应激和炎症反应,显示神经保护和镇痛特性,并且在遭受缺血再灌注损伤的器官或组织中具有保护功能。然而,目前尚无研究证实BCP能否应用于皮瓣移植以提高皮瓣成活率。
方法:为了评估BCP对随机皮瓣存活的影响,我们在大鼠上构建了改良的McFarlane随机皮瓣模型。用不同剂量的BCP连续灌胃7天后,我们测量了存活面积比,血管生成,血液灌注,组织炎症水平,凋亡相关蛋白水平,和PI3K/AKT信号通路在随机皮瓣中的表达。
结果:BCP处理以剂量依赖的方式增加了大鼠随机皮瓣移植后皮瓣的存活面积。BCP主要促进组织血管的形成,改善皮瓣血液灌注,限制了局部炎症反应,减少细胞凋亡。此外,我们证明BCP主要通过促进PI3K/AKT信号表达,同时增强AKT的磷酸化而起作用.沃特曼宁的管理,PI3K的选择性抑制剂,消除BCP的影响。
结论:BCP可通过上调PI3K/AKT信号通路促进随机皮瓣的存活,增加组织血液灌注,限制炎症反应和细胞凋亡。
BACKGROUND: Flap transplantation is a widely used plastic repair technique in surgical procedures, aimed at addressing skin defects resulting from diverse wounds and diseases. However, due to the insufficient blood supply after flap surgery, the occurrence of ischemia-reperfusion injury, and an excessive sterile inflammatory response, flaps frequently develop complications (e.g., partial or complete ischemic necrosis). These complications have adverse effects on wound healing and repair. β-Caryophyllene (BCP) is a bicyclic sesquiterpene that is widely present in plants. It mitigates oxidative stress and inflammatory responses, demonstrates neuroprotective and analgesic properties, and serves a protective function in organs or tissues subjected to ischemia-reperfusion injury. However, no study has confirmed whether BCP can be used in the field of flap transplantation to improve the flap survival rate.
METHODS: To assess the impact of BCP on random flap survival, we constructed a modified McFarlane random flap model on the rat. After 7 consecutive days of gavage with different doses of BCP, we measured the survival area ratio, angiogenesis, blood perfusion, tissue inflammation level, apoptosis-related protein levels, and the PI3K/AKT signaling pathway expression of the random flap.
RESULTS: BCP treatment increased the survival area of the flap in a dose-dependent manner after random flap transplantation in rats. BCP mainly promoted the formation of tissue blood vessels, improved flap blood perfusion, limited the local inflammatory response, and reduced apoptosis. In addition, we demonstrated that BCP works primarily by promoting the PI3K/AKT signaling expression while enhancing the phosphorylation of AKT. Administration of wortmannin, a selective inhibitor of PI3K, eliminated the effects of BCP.
CONCLUSIONS: BCP can promote the survival of random flaps by upregulating the PI3K/AKT signaling pathway, increasing tissue blood perfusion, and limiting the inflammatory response and apoptosis.