Abstract：
Mercury chloride (ME) is a chemical pollutant commonly found in the environment, which can contribute to undesirable health consequence worldwide. The current study investigated the detrimental impact of ME on the cerebellum and spinal cord tissues in 6-8-week-old female rats. We also evaluated the neuroprotective efficacy of β-caryophyllene (BC) against spinal and cerebellar changes caused by ME. Thirty-five young Wistar albino rats were randomly chosen and assigned into five groups: control (CO), olive oil (OI), ME, BC, ME + BC. All samples were analysed by means of unbiased stereological, biochemical, immunohistochemical, and histopathological methods. Our biochemical findings showed that SOD level was significantly increased in the ME group compared to the CO group (p < 0.05). We additionally detected a statistically significant decrease in the number of cerebellar Purkinje cells and granular cells, as well as spinal motor neuron in the ME group compared to the CO group (p < 0.05). In the ME + BC group, the number of Purkinje cells, granular cells, and spinal motor neurons was significantly higher compared to the ME group (p < 0.05). Decreased SOD activity in the ME + BC group was also detected than the ME group (p < 0.05). Immunohistochemical (the tumour necrosis factor-alpha (TNF-α)) and histopathological examinations also exhibited crucial information in each of the group. Taken together, ME exposure was associated with neurotoxicity in the cerebellum and spinal cord tissues. BC treatment also mitigated ME-induced neurological alteration, which may imply its potential therapeutic benefits.
摘要：
氯化汞（ME）是环境中常见的化学污染物，这可能导致全世界不良的健康后果。目前的研究调查了ME对6-8周龄雌性大鼠小脑和脊髓组织的有害影响。我们还评估了β-石竹烯（BC）对ME引起的脊髓和小脑变化的神经保护功效。随机选择35只年轻的Wistar白化病大鼠，并分为五组：对照组（CO），橄榄油(OI),我,BC,ME+BC。所有样本都通过无偏见的体视学分析，生物化学，免疫组织化学，和组织病理学方法。我们的生化发现表明，与CO组相比，ME组的SOD水平显着增加（p<0.05）。我们还检测到小脑浦肯野细胞和颗粒细胞数量的统计学显着减少，与CO组相比，ME组以及脊髓运动神经元（p<0.05）。在ME+BC组中，浦肯野细胞的数量，颗粒细胞,和脊髓运动神经元显著高于ME组(p<0.05)。ME+BC组的SOD活性也比ME组降低(p<0.05)。免疫组织化学（肿瘤坏死因子-α（TNF-α））和组织病理学检查在每组中也显示出重要信息。一起来看,ME暴露与小脑和脊髓组织的神经毒性有关。BC治疗也减轻了ME引起的神经改变，这可能意味着其潜在的治疗益处。
