目的:本研究旨在分析血浆醛固酮的分布,肾素活动,脱氧皮质酮(DOC),皮质醇,可的松,采用液相色谱-串联质谱(LC-MS/MS)法检测24h尿醛固酮(24h-uAld)水平。
方法:收集东北地区129名健康志愿者的血浆和24h尿液。钠摄入的影响,年龄,性别,采血时间对血浆醛固酮浓度(PAC),血浆肾素活性(PRA),PAC与PRA比率(ARR),DOC,皮质醇,可的松,皮质醇与可的松的比例,和24h-uAld通过非参数检验进行了研究,多元线性回归和Harris-Boyd标准偏差检验。
结果:在24h-uAld,PAC(AM),PRA(AM),ARR(AM),DOC(AM),皮质醇(AM),可的松(AM),高钠和低钠摄入组之间的皮质醇对可的松(AM)。上午和下午采样组在PAC方面观察到显著差异,ARR,DOC,皮质醇,和可的松.24h-uAld的参考间隔(RI),建议按性别划分PAC(AM)。PRA的RI建议进行年龄分层。
结论:我们建议无论钠摄入量如何,都可以使用相同的参考区间。性别是24h-uAld的主要影响因素,PAC,ARR。年龄是PRA的关键影响因素。
OBJECTIVE: This study aims to analyze the distribution of plasma aldosterone, renin activity, deoxycorticosterone (DOC), cortisol, cortisone, and 24 h urinary aldosterone (24 h-uAld) levels based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) method.
METHODS: Plasma and 24 h urine were collected from 129 healthy volunteers in Northeast China. The effect of sodium intake, age, gender, blood sampling time on plasma aldosterone concentration (PAC), plasma renin activity (PRA), PAC to PRA ratio (ARR), DOC, cortisol, cortisone, cortisol to cortisone ratio, and 24 h-uAld were investigated by nonparametric test, multiple linear regression and Harris-Boyd\'s standard deviate test.
RESULTS: There was no significant difference observed in 24 h-uAld, PAC (AM), PRA(AM), ARR (AM), DOC (AM), cortisol (AM), cortisone (AM), and cortisol to cortisone (AM) between high and low sodium intake group. Significant differences were observed between morning and afternoon sampling groups in terms of PAC, ARR, DOC, cortisol, and cortisone. Reference intervals (RIs) of 24 h-uAld, PAC (AM) were recommended to be partitioned by gender. RI of PRA was recommended age stratification.
CONCLUSIONS: We recommend that the same reference interval could be used regardless of sodium intake. Gender is the main influence factor for 24 h-uAld, PAC, and ARR. Age is key influence factor for PRA.