Abstract：
Proteins are essential components of human cells and tissues, and they are commonly measured in clinical laboratories using immunoassays. However, these assays have certain limitations, such as non-specificity binding, insufficient selectivity, and interference of antibodies. More sensitive, accurate, and efficient technology is required to overcome these limitations. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful analytical tool that provides high sensitivity and specificity, making it superior to traditional methods such as biochemical methods and immunoassays. While LC-MS/MS has been increasingly used for detecting small molecular analytes and steroid hormones in clinical practice recently, its application for protein or peptide analysis is still in its early stages. Established methods for quantifying proteins and peptides by LC-MS/MS are mainly focused on scientific research, and only a few proteins and peptides can be or have the potential to be detected and applied in clinical practice. Therefore, this article aims to review the clinical applications, advantages, and challenges of analyzing proteins and peptides using LC-MS/MS in clinical laboratories.
摘要：
蛋白质是人类细胞和组织的重要组成部分，它们通常在临床实验室使用免疫测定法进行测量。然而,这些检测方法有一定的局限性,如非特异性结合，选择性不足,和抗体的干扰。更敏感,准确,并且需要有效的技术来克服这些限制。液相色谱-串联质谱（LC-MS/MS）是一种功能强大的分析工具，可提供高灵敏度和特异性，使其优于传统的方法，如生化和免疫测定。虽然LC-MS/MS近年来在临床实践中越来越多地用于检测小分子分析物和类固醇激素，它在蛋白质或肽分析中的应用仍处于早期阶段。建立的LC-MS/MS定量蛋白质和多肽的方法主要集中在科学研究,只有少数蛋白质和肽可以或具有潜力被检测和应用于临床实践。因此,本文旨在回顾其临床应用，优势,以及在临床实验室中使用LC-MS/MS分析蛋白质和肽的挑战。
