■确定负责对热应激反应的分子机制对于增加产量至关重要,繁殖,健康,和福利。本研究旨在通过血浆蛋白质组学分析,确定热带适应肉牛对热应激和动物恢复的早期生物学反应和潜在生物标志物。
■根据湿球温度指数和直肠温度评估,在热应激高峰(HSP)期间和之后16小时(热应激恢复-HSR)从14名加拉州男性中收集血液样本。通过液相色谱-串联质谱法从血浆样品中研究蛋白质组,并使用DAVID工具通过功能富集分析评估差异调节的蛋白质。通过STRING工具评估蛋白质-蛋白质相互作用网络。
■在两个时间点共检测到1,550种蛋白质,其中84和65在高铁期间下调和上调,分别。在具有最高绝对对数倍数变化值的差异调节蛋白中,由GABBR1,EPHA2,DUSP5,MUC2,DGCR8,MAP2K7,ADRA1A,CXADR,TOPBP1和NEB基因被强调为潜在的生物标志物,因为它们在响应热应激中的作用。功能富集分析显示,65个基因本体术语和34个通路显著(P<0.05)。我们强调了可能与对热应激的反应有关的那些,比如与免疫系统有关的,互补系统,止血,钙,ECM-受体相互作用,以及PI3K-Akt和MAPK信号通路。此外,蛋白质-蛋白质相互作用网络分析显示,基于其中心性和边缘,几种补体和凝血蛋白以及急性期蛋白是重要的节点。
■识别差异调节蛋白及其关系,以及它们在关键途径中的作用有助于提高对自然适应的牛品种对热应激反应背后的机制的认识。此外,本文强调的蛋白质是参与热带适应肉牛的早期响应和热应激恢复的潜在生物标志物。
UNASSIGNED: Identifying molecular mechanisms responsible for the response to heat stress is essential to increase production, reproduction, health, and welfare. This study aimed to identify early biological responses and potential biomarkers involved in the response to heat stress and animal\'s recovery in tropically adapted beef cattle through proteomic analysis of blood plasma.
UNASSIGNED: Blood samples were collected from 14 Caracu males during the heat stress peak (HSP) and 16 h after it (heat stress recovery-HSR) assessed based on wet bulb globe temperature index and rectal temperature. Proteome was investigated by liquid chromatography-tandem mass spectrometry from plasma samples, and the differentially regulated proteins were evaluated by functional enrichment analysis using DAVID tool. The protein-protein interaction network was evaluated by STRING tool.
UNASSIGNED: A total of 1,550 proteins were detected in both time points, of which 84 and 65 were downregulated and upregulated during HSR, respectively. Among the differentially regulated proteins with the highest absolute log-fold change values, those encoded by the GABBR1, EPHA2, DUSP5, MUC2, DGCR8, MAP2K7, ADRA1A, CXADR, TOPBP1, and NEB genes were highlighted as potential biomarkers because of their roles in response to heat stress. The functional enrichment analysis revealed that 65 Gene Ontology terms and 34 pathways were significant (P < 0.05). We highlighted those that could be associated with the response to heat stress, such as those related to the immune system, complement system, hemostasis, calcium, ECM-receptor interaction, and PI3K-Akt and MAPK signaling pathways. In addition, the protein-protein interaction network analysis revealed several complement and coagulation proteins and acute-phase proteins as important nodes based on their centrality and edges.
UNASSIGNED: Identifying differentially regulated proteins and their relationship, as well as their roles in key pathways contribute to improve the knowledge of the mechanisms behind the response to heat stress in naturally adapted cattle breeds. In addition, proteins highlighted herein are potential biomarkers involved in the early response and recovery from heat stress in tropically adapted beef cattle.