液相色谱-串联质谱(LC-MS/MS)越来越多地用于临床实验室,因为它在特异性和灵敏度方面优于其他分析技术。鉴于许多测定和平台不作为单个试剂盒或装置提供给实验室,这些优点伴随着额外的责任和挑战。技能,工作人员,LC-MS/MS中使用的测定法由实验室内部开发,相对较少的例外。因此,部署LC-MS/MS测定的实验室必须认真考虑为克服与该技术相关的挑战而采用的实践和程序。这篇综述讨论了LC-MS/MS测定法的开发后前景,包括验证,质量保证,操作,和故障排除。LC-MS/MS用户的内容知识相当广泛和深入,涵盖多个科学领域,包括生物学,临床化学,色谱,工程,和女士然而,除了方法开发报告之外,没有正式的学术课程或特定的文献来培训实验室人员了解LC-MS/MS的基础知识。因此,根据他们的经验水平,一些读者可能熟悉这里描述的实验室实践的各个方面,而其他人可能不是。本综述致力于在临床实验室中组装LC-MS/MS操作的各个方面,以为周到地开发和执行LC-MS/MS应用程序提供框架。
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly utilized in clinical laboratories because it has advantages in terms of specificity and sensitivity over other analytical technologies. These advantages come with additional responsibilities and challenges given that many assays and platforms are not provided to laboratories as a single kit or device. The skills, staff, and assays used in LC-MS/MS are internally developed by the laboratory, with relatively few exceptions. Hence, a laboratory that deploys LC-MS/MS assays must be conscientious of the practices and procedures adopted to overcome the challenges associated with the technology. This
review discusses the post-development landscape of LC-MS/MS assays, including validation, quality assurance, operations, and troubleshooting. The content knowledge of LC-MS/MS users is quite broad and deep and spans multiple scientific fields, including biology, clinical chemistry, chromatography, engineering, and MS. However, there are no formal academic programs or specific literature to train laboratory staff on the fundamentals of LC-MS/MS beyond the reports on method development. Therefore, depending on their experience level, some readers may be familiar with aspects of the laboratory practices described herein, while others may be not. This
review endeavors to assemble aspects of LC-MS/MS operations in the clinical laboratory to provide a framework for the thoughtful development and execution of LC-MS/MS applications.