使用多相分类学方法来表征这三种细菌菌株(FP830T,FP2034和FP2262)从水稻的根际土壤中分离,玉米,还有北京的高原大麦,黑龙江,西藏,分别,在PR中国。这些菌株是革兰氏阴性的,杆状,并有一个或两个极地鞭毛。在1%(w/v)NaCl存在下,它们在28°C和pH7.0下表现出最佳生长,并且在King'sB平板上培养时在紫外线下显示荧光。FP830T基因组大小为6.4Mbp,G+C含量为61.0mol%。FP830T具有通过产生各种代谢产物如fengycin来促进植物生长的潜力,pyoverdin,吲哚-3-乙酸,和挥发性物质2,3-丁二醇。系统发育分析表明,三个分离株形成了一个独立的分支,与菌株DSM13194T和zanjanenensisSWRI12T最密切相关。三个分离株和近亲之间的平均核苷酸同一性和数字DNA-DNA杂交值不高于93.7和52.3%,分别。主要的细胞脂肪酸是C16:0,总特征3(C16:1ω7c/C16:1ω6c),和求和特征8(C18:1ω7c/C18:1ω6c)。主要极性脂质是磷脂酰乙醇胺,二磷脂酰甘油,和氨基磷脂.主要的呼吸醌是泛醌(Q-9)。基于多相分类分析,结论是菌株FP830T,FP2034和FP2262代表假单胞菌属中的一个新物种,和北京假单胞菌。11月。被提议命名为新物种。菌株类型为FP830T(=ACCC62448T=JCM35689T)。
A polyphasic taxonomic approach was used to characterize the three bacterial strains (FP830T, FP2034, and FP2262) isolated from the rhizosphere soil of rice, corn, and highland barley in Beijing, Heilongjiang, and Tibet, respectively, in PR China. These strains were Gram-negative, rod-shaped, and have one or two polar flagella. They exhibited optimal growth at 28 °C and pH 7.0 in the presence of 1 % (w/v) NaCl and showed fluorescence under ultraviolet light when cultivated on King\'s B plates. The FP830T genome size is 6.4 Mbp with a G+C content of 61.0 mol%. FP830T has the potential to promote plant growth by producing various metabolites such as fengycin, pyoverdin, indole-3-acetic acid, and the volatile substance 2,3-butanediol. Phylogenetic analysis indicated that three isolates formed an independent branch, which most closely related to type strains Pseudomonas thivervalensis DSM 13194T and Pseudomonas zanjanensis SWRI12T. The values of average nucleotide identity and digital DNA-DNA hybridization between three isolates and closest relatives were not higher than 93.7 and 52.3 %, respectively. The dominant cellular fatty acids were C16 : 0, summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c), and summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and aminophospholipid. The predominant respiratory quinone was ubiquinone (Q-9). Based on polyphasic taxonomic analysis, it was concluded that strains FP830T, FP2034, and FP2262 represented a novel species within the genus Pseudomonas, and Pseudomonas beijingensis sp. nov. was proposed for the name of novel species. The type strain is FP830T (=ACCC 62448T=JCM 35689T).