Abstract：
A Gram-stain-negative, light khaki, strictly aerobic, rod-shaped, motile via multiple flagella, and catalase- and oxidase-positive bacterium, designated as SSM4.3T, was isolated from the seaweed of Gouqi Island in the East China Sea. The novel isolate grows at 0-5.0% NaCl concentrations (w/v) (optimum 1%), pH 5.0-9.0 (optimum pH 7.0), and 15-37 °C (optimum 30 °C). The 16S rRNA gene sequences-based phylogeny indicates that the novel marine isolate belongs to the family Rhizobiaceae and that it shared the greatest sequence similarity (98.9%) with Peteryoungia rhizophila CGMCC 1.15691T. This classification was also supported by phylogenetic analysis using core genes. The predominant fatty acids (≥ 10%) of the strain were identified as C18:1 ω7c/C18:1 ω6c. Q-10 was identified as the major isoprenoid quinone, with trace levels of Q-9 present. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The complete genome size of strain SSM4.3T is 4.39 Mb with a DNA G+C content of 61.3%. The average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values between the genomes of strain SSM4.3T and its closely related representatives were 74.80-86.93%, 20.00-32.30%, and 70.30-91.52%, respectively. Phylogenetic analysis, grounded on the core genes, reveals the evolutionary relationship between SSM4.3T and other Peteryoungia strains. Pan-genomics analysis of 8 previously classified Peteryoungia species and SSM4.3T revealed their unique genetic features and functions. Overall, strain SSM4.3T was considered to be a new species of the Peteryoungia genus; the name Peteryoungia algae sp. nov. has been proposed, with type strain SSM4.3T (= LMG 32561 = MCCC 1K07170).
摘要：
革兰氏染色阴性，浅卡其色,严格的有氧，杆状,通过多个鞭毛运动，过氧化氢酶和氧化酶阳性细菌，指定为SSM4.3T，是从东海沟旗岛的海藻中分离出来的。新型分离物在0-5.0％NaCl浓度（w/v）（最佳1％）下生长，pH5.0-9.0（最佳pH7.0），和15-37°C（最佳30°C）。基于16SrRNA基因序列的系统发育表明，该新型海洋分离株属于根瘤菌科，与PeteryoungiarhighzophilaCGMCC1.15691T具有最大的序列相似性（98.9％）。这种分类也得到了使用核心基因的系统发育分析的支持。该菌株的主要脂肪酸(≥10%)被鉴定为C18:1ω7c/C18:1ω6c。Q-10被确定为主要的类异戊二烯醌，有微量的Q-9存在。主要极性脂质被鉴定为二磷脂酰甘油，磷脂酰乙醇胺和磷脂酰甘油。菌株SSM4.3T的完整基因组大小为4.39Mb，DNAGC含量为61.3％。平均核苷酸同一性，数字DNA-DNA杂交，菌株SSM4.3T及其密切相关的代表的基因组之间的平均氨基酸同一性值为74.80-86.93％，20.00-32.30%，和70.30-91.52%，分别。系统发育分析,以核心基因为基础，揭示了SSM4.3T与其他Peteryoungia菌株之间的进化关系。对8种先前分类的Peteryoungia物种和SSM4.3T进行的泛基因组学分析揭示了它们独特的遗传特征和功能。总的来说,菌株SSM4.3T被认为是Peteryoungia属的新物种；名称为Peteryoungia藻类sp。11月。已经被提议，与SSM4.3T型菌株（=LMG32561=MCCC1K07170）。
