Abstract:
A Gram-stain-negative, light khaki, strictly aerobic, rod-shaped, motile via multiple flagella, and catalase- and oxidase-positive bacterium, designated as SSM4.3T, was isolated from the seaweed of Gouqi Island in the East China Sea. The novel isolate grows at 0-5.0% NaCl concentrations (w/v) (optimum 1%), pH 5.0-9.0 (optimum pH 7.0), and 15-37 °C (optimum 30 °C). The 16S rRNA gene sequences-based phylogeny indicates that the novel marine isolate belongs to the family Rhizobiaceae and that it shared the greatest sequence similarity (98.9%) with Peteryoungia rhizophila CGMCC 1.15691T. This classification was also supported by phylogenetic analysis using core genes. The predominant fatty acids (≥ 10%) of the strain were identified as C18:1 ω7c/C18:1 ω6c. Q-10 was identified as the major isoprenoid quinone, with trace levels of Q-9 present. The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The complete genome size of strain SSM4.3T is 4.39 Mb with a DNA G+C content of 61.3%. The average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values between the genomes of strain SSM4.3T and its closely related representatives were 74.80-86.93%, 20.00-32.30%, and 70.30-91.52%, respectively. Phylogenetic analysis, grounded on the core genes, reveals the evolutionary relationship between SSM4.3T and other Peteryoungia strains. Pan-genomics analysis of 8 previously classified Peteryoungia species and SSM4.3T revealed their unique genetic features and functions. Overall, strain SSM4.3T was considered to be a new species of the Peteryoungia genus; the name Peteryoungia algae sp. nov. has been proposed, with type strain SSM4.3T (= LMG 32561 = MCCC 1K07170).
摘要:
革兰氏染色阴性,浅卡其色,严格的有氧,杆状,通过多个鞭毛运动,过氧化氢酶和氧化酶阳性细菌,指定为SSM4.3T,是从东海沟旗岛的海藻中分离出来的。新型分离物在0-5.0%NaCl浓度(w/v)(最佳1%)下生长,pH5.0-9.0(最佳pH7.0),和15-37°C(最佳30°C)。基于16SrRNA基因序列的系统发育表明,该新型海洋分离株属于根瘤菌科,与PeteryoungiarhighzophilaCGMCC1.15691T具有最大的序列相似性(98.9%)。这种分类也得到了使用核心基因的系统发育分析的支持。该菌株的主要脂肪酸(≥10%)被鉴定为C18:1ω7c/C18:1ω6c。Q-10被确定为主要的类异戊二烯醌,有微量的Q-9存在。主要极性脂质被鉴定为二磷脂酰甘油,磷脂酰乙醇胺和磷脂酰甘油。菌株SSM4.3T的完整基因组大小为4.39Mb,DNAGC含量为61.3%。平均核苷酸同一性,数字DNA-DNA杂交,菌株SSM4.3T及其密切相关的代表的基因组之间的平均氨基酸同一性值为74.80-86.93%,20.00-32.30%,和70.30-91.52%,分别。系统发育分析,以核心基因为基础,揭示了SSM4.3T与其他Peteryoungia菌株之间的进化关系。对8种先前分类的Peteryoungia物种和SSM4.3T进行的泛基因组学分析揭示了它们独特的遗传特征和功能。总的来说,菌株SSM4.3T被认为是Peteryoungia属的新物种;名称为Peteryoungia藻类sp。11月。已经被提议,与SSM4.3T型菌株(=LMG32561=MCCC1K07170)。