■表达绿色荧光蛋白(GFP)的转基因裸鼠,红色荧光蛋白(RFP),或青色荧光蛋白(CFP)以前是由我们的实验室开发的,AntiCancerInc.在本研究中,我们展示了GFP的成像,RFP,或CFP裸鼠用单一仪器进行单纳米调谐激光荧光激发。
■雌性转基因C57/B6裸GFP,RFP,使用6周龄的CFP小鼠。使用UVP生物光谱高级系统(AnalytikJenaUSLLC)获得图像,在480nm激发,GFP的峰值发射在513nm;520nm和605nm,分别,对于RFP;以及405nm和480nm,分别,CFP。
■对于每只彩色转基因荧光小鼠,没有背景的图像可以单独获得与UVP生物频谱先进的系统。
■使用一台仪器,灿烂和明确的GFP图像,RFP,采用单纳米调谐激光荧光激发获得CFP小鼠。该成像系统将在未来的研究中用于分析光谱不同的有色小鼠中的癌细胞,以确定基质细胞和癌症如何在肿瘤微环境中相互作用。
UNASSIGNED: Transgenic nude mice expressing green fluorescent protein (
GFP), red fluorescent protein (RFP), or cyan fluorescent protein (CFP) were previously developed by our laboratory, AntiCancer Inc. In the present study, we demonstrate imaging of the
GFP, RFP, or CFP nude mice with single-nanometer-tuning laser fluorescence excitation with a single instrument.
UNASSIGNED: Female transgenic C57/B6 nude
GFP, RFP, and CFP mice aged six weeks were used. The images were obtained using the UVP Biospectrum Advanced system (Analytik Jena US LLC) with excitation at 480 nm and peak emission at 513 nm for
GFP; 520 nm and 605 nm, respectively, for RFP; and 405 nm and 480 nm, respectively, for CFP.
UNASSIGNED: For each color transgenic fluorescent mouse, images without background could be obtained individually with the UVP Biospectrum Advanced system.
UNASSIGNED: Using a single instrument, brilliant and well-defined images of
GFP, RFP, and CFP mice were obtained with single-nanometer-tuning laser fluorescence excitation. This imaging system will be used in future studies to analyze cancer cells in the colored mice that are spectrally distinct in order to determine how stromal cells and cancer interact in the tumor microenvironment.