关键词: Actin waves Cell fusion GFP Gradient sensing PIP3 PTEN RFP Ras

Mesh : Dictyostelium / physiology cytology Chemotaxis Chemotactic Factors / pharmacology metabolism Actins / metabolism Cell Fusion / methods Giant Cells / cytology metabolism Cell Polarity

来  源:   DOI:10.1007/978-1-0716-4023-4_12

Abstract:
Normal-sized cells of Dictyostelium build up a front-tail polarity when they respond to a gradient of chemoattractant. To challenge the polarity-generating system, cells are fused to study the chemotactic response of oversized cells that extend multiple fronts toward the source of attractant. An aspect that can be explored in these cells is the relationship of spontaneously generated actin waves to actin reorganization in response to chemoattractant.
摘要:
当它们对化学引诱物的梯度做出反应时,正常大小的网藻菌类细胞会形成前尾极性。为了挑战极性产生系统,融合细胞以研究超大细胞的趋化反应,这些细胞向引诱剂来源延伸多个前沿。可以在这些细胞中探索的一个方面是响应于化学引诱物自发产生的肌动蛋白波与肌动蛋白重组的关系。
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