%0 Journal Article
%T A self-inducible heterologous protein expression system in Komagataella phaffii (Pichia pastoris).
%A Unver Y
%A Ari B
%A Acar M
%A Yildiz Arslan S
%J 3 Biotech
%V 14
%N 9
%D 2024 Sep
%M 39131177
%F 2.893
%R 10.1007/s13205-024-04039-x
%X Komagataella phaffii (previously described as Pichia pastoris) is a yeast that produces high-level heterologous proteins with a wide range of applications in medicine and industry. The methanol-induced alcohol oxidase I promoter (PAOX1) is frequently used for protein expression in this yeast. However, limitations on the use of methanol have been observed in large-scale production, including its flammability, toxicity, and need for special handling. Here, we propose to develop a system using recombinant cells constitutively expressing pectinmethyl esterase for expression of two reporter proteins, GFP and azurin, under the control of PAOX1 using pectin in production medium. So, this system is coherent with yeast culture medium containing pectin and heterologous gene inserted downstream of PAOX1 can be successfully expressed without the addition of methanol. Therefore, this novel Self-inducibLe heterologous protein EXpression (SILEX) system, which does not require the addition of methanol, can be used for the production of any protein. It can also be adapted for large-scale production.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-024-04039-x.