■脾脏在免疫反应调节中起着必不可少的作用,被认为是最大的外周免疫器官。胞二磷胆碱用于各种脑部疾病的管理。本研究旨在探讨胞磷胆碱治疗γ-辐射诱导的大鼠脾炎症的可能性。
将18只雄性白化病大鼠分类为:第1组(对照)动物作为对照。第2组(γ辐射)动物用6Gy进行全身γ辐射。第3组(γ射线+胞磷胆碱)大鼠用6Gyγ射线照射,照射后5min,腹腔注射胞磷胆碱(300mg/kg/d),持续一周。TNF-α水平,IL-1β,iNOS,NF-κB,在脾组织中测定JAK2和STAT3,以及组织病理学检查。
■大鼠暴露于γ辐射导致脾脏TNF-α升高,IL-1β,NF-κB,iNOS,JAK2和STAT3水平显著。γ辐射暴露后用胞二磷胆碱治疗改善了这种升高,和调制的γ辐射诱导的组织病理学改变。
■这些数据表明胞磷胆碱通过抑制脾组织中的NF-κB和JAK2/STAT3信号通路抑制γ-辐射诱导的脾炎症。
UNASSIGNED: The spleen has an essential role in immune responses regulation and is considered the biggest peripheral immune organ. Citicoline is used for various brain disorders management. This study aimed to examine the using possibility of citicoline to treat γ-radiation-induced splenic inflammation in rats.
UNASSIGNED: Eighteen male albino rats were classified into: Group 1 (control) animals were kept as control. Group 2 (γ-radiation) animals were total-body γ-irradiated with 6 Gy. Group 3 (γ-radiation + citicoline) rats were γ-irradiated with 6 Gy, then injected intraperitoneally with citicoline (300 mg/kg/d) 5 min after irradiation for one week. Levels of TNF-α, IL-1β, iNOS, NF-κB, JAK2, and STAT3 were determined in spleen tissue, along with histopathological examination.
UNASSIGNED: Rats exposure to gamma-radiation led to elevation in splenic TNF-α, IL-1β, NF-κB, iNOS, JAK2, and STAT3 levels significantly. Treatment with citicoline after gamma-radiation exposure improved this elevation, and modulated gamma-radiation-induced histopathological alterations.
UNASSIGNED: This data showed that citicoline inhibited γ-radiation-induced splenic inflammation via suppressing NF-κB and JAK2/STAT3 signaling pathways in spleen tissue.