缺氧是水生生态系统中常见的环境胁迫源,在培养过程中,巨齿斑病容易死亡,因为它不耐受缺氧,给农民带来巨大的经济损失。脑垂体是鱼类的重要内分泌腺,它主要参与分泌,storage,和激素的调节。在本研究中,我们比较了缺氧敏感(HS)和缺氧耐受(HT)的脑垂体对严重缺氧的转录反应。根据缺氧处理期间失去平衡所需的时间对鱼进行分类。总共获得129,251,170个原始读数。原始序列过滤后,HS获得了43,461,745、42,609,567和42,730,282个干净的读数,HT,和C0组,分别。转录组比较揭示了在C0与C0中差异表达的1234个基因。HS,虽然获得了C0与C0的1646个差异表达基因HT。此外,HS与HS的结果HT显示,对于总共408个差异表达基因,获得了367个上调和41个下调的差异表达基因。C0与C0的KEGG分析HS,C0vs.HT,和HSvs.HT确定了315、322和219个富集的途径,分别。类似的缺氧诱导的转录模式表明,下调的DEGs和富集的通路与多种疾病的神经变性通路有关。癌症的通路,产热,microRNAs在癌症中,糖尿病性心肌病,和肾素分泌。然而,在上调的DEG中,PI3K-Akt信号通路(C0vs.HS),癌症中的microRNAs(C0vs.HT),和HIF-1信号通路(HSvs.HT)显著富集。关于垂体在低氧应激中的作用尚不清楚。这些结果不仅为脑垂体组织应对缺氧应激的机制提供了新的见解,而且为培育具有耐缺氧性状的小脑提供了基础。
Hypoxia is a common environmental stressor in aquatic ecosystems, and during the cultivation process, Megalobrama amblycephala is prone to death because it is hypoxia-intolerant, which brings huge economic losses to farmers. The pituitary gland is a crucial endocrine gland in fish, and it is mainly involved in the secretion, storage, and regulation of hormones. In the present study, we compared the transcriptional responses to serious hypoxia in the pituitary gland among hypoxia-sensitive (HS) and hypoxia-tolerant (HT) M. amblycephala and a control group that received a normal oxygen supply (C0). The fish were categorized according to the time required to lose balance during a hypoxia treatment. A total of 129,251,170 raw reads were obtained. After raw sequence filtering, 43,461,745, 42,609,567, and 42,730,282 clean reads were obtained for the HS, HT, and C0 groups, respectively. A
transcriptomic comparison revealed 1234 genes that were differentially expressed in C0 vs. HS, while 1646 differentially expressed genes were obtained for C0 vs. HT. In addition, the results for HS vs. HT showed that 367 upregulated and 41 downregulated differentially expressed genes were obtained for a total of 408 differentially expressed genes. A KEGG analysis of C0 vs. HS, C0 vs. HT, and HS vs. HT identified 315, 322, and 219 enriched pathways, respectively. Similar hypoxia-induced transcription patterns suggested that the downregulated DEGs and enriched pathways were related to pathways of neurodegeneration in multiple diseases, pathways in cancer, thermogenesis, microRNAs in cancer, diabetic cardiomyopathy, and renin secretion. However, in the upregulated DEGs, the PI3K-Akt signaling pathway (C0 vs. HS), microRNAs in cancer (C0 vs. HT), and HIF-1 signaling pathway (HS vs. HT) were significantly enriched. There is a lack of clarity regarding the role of the pituitary gland in hypoxic stress. These results not only provide new insights into the mechanism by which pituitary tissue copes with hypoxia stress in M. amblycephala but also offer a basis for breeding M. amblycephala with hypoxia-resistant traits.