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Abstract：
High temperature stress is one of the most severe forms of abiotic stress in alfalfa. With the intensification of climate change, the frequency of high temperature stress will further increase in the future, which will bring challenges to the growth and development of alfalfa. Therefore, untargeted metabolomic and RNA-Seq profiling were implemented to unravel the possible alteration in alfalfa seedlings subjected to different temperature stress (25 ℃, 30 ℃, 35 ℃, 40 ℃) in this study. Results revealed that High temperature stress significantly altered some pivotal transcripts and metabolites. The number of differentially expressed genes (DEGs) markedly up and down-regulated was 1876 and 1524 in T30_vs_CK, 2, 815 and 2667 in T35_vs_CK, and 2115 and 2, 226 in T40_vs_CK, respectively. The number for significantly up-regulated and down-regulated differential metabolites was 173 and 73 in T30_vs_CK, 188 and 57 in T35_vs_CK, and 220 and 66 in T40_vs_CK, respectively. It is worth noting that metabolomics and transcriptomics co-analysis characterized enriched in plant hormone signal transduction (ko04705), glyoxylate and dicarboxylate metabolism (ko00630), from which some differentially expressed genes and differential metabolites participated. In particular, the content of hormone changed significantly under T40 stress, suggesting that maintaining normal hormone synthesis and metabolism may be an important way to improve the HTS tolerance of alfalfa. The qRT-PCR further showed that the expression pattern was similar to the expression abundance in the transcriptome. This study provides a practical and in-depth perspective from transcriptomics and metabolomics in investigating the effects conferred by temperature on plant growth and development, which provided the theoretical basis for breeding heat-resistant alfalfa.
摘要：
高温胁迫是紫花苜蓿中最严重的非生物胁迫形式之一。随着气候变化的加剧,未来高温应力的频率将进一步增加，这将给苜蓿的生长发育带来挑战。因此,实施了非靶向代谢组学和RNA-Seq分析，以揭示受不同温度胁迫（25℃，30℃,35℃,40℃)在这项研究中。结果表明，高温胁迫显着改变了一些关键转录本和代谢产物。在T30_vs_CK中，差异表达基因（DEGs）明显上调和下调的数量分别为1876和1524，T35_vs_CK中的2、815和2667，和2115和2，226在T40_vs_CK中，分别。在T30_vs_CK中，显著上调和下调的差异代谢物的数量分别为173和73，T35_vs_CK中的188和57，T40_vs_CK中的220和66，分别。值得注意的是，代谢组学和转录组学联合分析的特征在于植物激素信号转导（ko04705），乙醛酸和二羧酸代谢（ko00630），其中一些差异表达基因和差异代谢产物参与其中。特别是,在T40胁迫下，激素含量发生显著变化，提示维持正常的激素合成和代谢可能是提高苜蓿对HTS耐受性的重要途径。qRT-PCR进一步显示表达模式与转录组中的表达丰度相似。本研究从转录组学和代谢组学的角度研究温度对植物生长和发育的影响，为培育耐高温苜蓿提供了理论依据。
