PDF(Pubmed)
Abstract：
Infections caused by fungi are emerging global health challenges that are exacerbated by the formation of fungal biofilms. Further challenges arise from environmental contamination with antifungal agents, which promotes environmental acquisition of antifungal resistance. We report the generation of an efficient, sustainable, all-natural antifungal nanotherapeutic based on the integration of an antimicrobial natural essential oil into a gelatin-based nanoemulsion platform. Carvacrol-loaded gelatin nanoemulsions penetrated Candida albicans biofilms, resulting in death of C. albicans cells in biofilms, and displayed selective biofilm elimination without harmful effects on fibroblast cells in a fungal biofilm-mammalian fibroblast co-culture model. Furthermore, the nanoemulsions degraded in the presence of physiologically relevant biomolecules, reducing the potential for environmental pollution and ecotoxicity. Overall, the sustainability, and efficacy of the described gelatin nanoemulsion formulation provides an environmentally friendly strategy for treating biofilm-associated fungal infections, including those caused by drug-resistant fungi.
摘要：
由真菌引起的感染正在出现的全球健康挑战，真菌生物膜的形成加剧了这种挑战。进一步的挑战来自抗真菌剂的环境污染，促进环境获得抗真菌抗性。我们报告了一种高效的，可持续,基于将抗菌天然精油整合到基于明胶的纳米乳液平台中的全天然抗真菌纳米治疗剂。加载香芹酚的明胶纳米乳液渗透白色念珠菌生物膜，导致生物膜中的白色念珠菌细胞死亡，并在真菌生物膜-哺乳动物成纤维细胞共培养模型中显示出选择性的生物膜消除，对成纤维细胞没有有害影响。此外，纳米乳液在生理相关生物分子的存在下降解，减少潜在的环境污染和生态毒性。总的来说,可持续性,和功效的所述明胶纳米乳液制剂提供了一种环境友好的策略，用于治疗生物膜相关的真菌感染，包括由耐药真菌引起的。
