PDF(Pubmed)
Abstract：
Cuscuta chinensis (C. chinensis) presents many pharmacological activities, including antidiabetic effects, and antioxidant, anti-inflammatory, and antitumor properties. However, the wound care properties of this plant have not yet been reported. Therefore, this research aimed to evaluate the antioxidant, anti-inflammatory, and antibacterial activities of ethanol and ethyl acetate C. chinensis extracts. The phytochemical markers in the extracts were analyzed using high-performance liquid chromatography (HPLC). Then, the selected C. chinensis extract was developed into a nanoemulsion-based gel for wound care testing in rats. The results showed that both of the C. chinensis extracts exhibited antioxidant activity when tested using 2,2-Diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and lipid peroxidation inhibition assays. They reduced the expression of IL-1β, IL-6, and TNF-α in RAW264.7 cells induced with lipopolysaccharide (LPS). The ethyl acetate extract also had antibacterial properties. Kaempferol was found in both extracts, whereas hyperoside was found only in the ethanol extract. These compounds were found to be related to the biological activities of the extracts, confirmed via molecular docking. The C. chinensis extract-loaded nanoemulsions had a small particle size, a narrow polydispersity index (PDI), and good stability. Furthermore, the C. chinensis extract-loaded nanoemulsion-based gel had a positive effect on wound healing, presenting a better percentage wound contraction Fucidin cream. In conclusion, this formulation has the potential for use as an alternative wound treatment and warrants further study in clinical trials.
摘要：
Cuscutachinensis(C.中国）具有许多药理活性，包括抗糖尿病作用，和抗氧化剂，抗炎,和抗肿瘤特性。然而,这种植物的伤口护理特性尚未报道。因此,这项研究旨在评估抗氧化剂，抗炎,乙醇和乙酸乙酯的抑菌活性。使用高效液相色谱（HPLC）分析提取物中的植物化学标记。然后，选定的C.chinensis提取物被开发成基于纳米乳液的凝胶，用于大鼠的伤口护理测试。结果表明，当使用2,2-二苯基-1-吡啶酰肼（DPPH）测试时，两种C.chinensis提取物均表现出抗氧化活性，三价铁还原抗氧化能力(FRAP)，和脂质过氧化抑制试验。他们降低了IL-1β的表达，脂多糖(LPS)诱导RAW264.7细胞中的IL-6和TNF-α。乙酸乙酯提取物也具有抗菌性能。在两种提取物中都发现了山奈酚，而金丝桃苷仅在乙醇提取物中发现。发现这些化合物与提取物的生物活性有关，通过分子对接确认。C.chinensis提取物负载的纳米乳液具有小的粒径，窄的多分散指数(PDI)，和良好的稳定性。此外，C.chinensis提取物纳米乳基凝胶对伤口愈合有积极作用，呈现出更好的伤口收缩百分比Fucidin乳膏。总之,该制剂具有作为替代伤口治疗方法的潜力,值得在临床试验中进一步研究.
