■牙周炎的早期症状较轻,这使得诊断成问题。牙龈上皮可用于微核试验,因为牙龈是受疾病影响的区域。
■该研究的目的是研究健康人和牙周炎患者牙龈上皮核异常的发生频率和范围。
■在对照组和实验组(10名35-50岁的健康男性和10名患有牙周炎的男性)中,在切牙(1.1)和磨牙(1.7)旁边刮除牙龈上皮。
■制剂用Romanowsky-Giemsa染色。核畸变的频率(‰),积累指数,并确定了修复指数。
■使用Wilcoxon和Van-der-Waerden试验确定了核畸变的中位数差异。使用Z检验比较病理比例。为了确定牙周炎的预测因子,采用接收机算子特性分析。对于多重比较,使用Bonferroni校正。
■在实验组中,核畸变的范围更广,未受影响区域的核溶解率较高,比对照组少;受影响区域的核周液泡较少,大核较多。患区微核超过1.33‰的细胞频率是牙周炎的标志。
■牙龈上皮可用于微核试验。微核试验显示,与健康受试者相比,牙周病患者牙龈上皮细胞的核畸变范围更广,微核的发生频率更高。因此,炎症的细胞学征象比临床征象更早出现,并且得到更清楚的证实。细胞凋亡和细胞核破坏的标志物,低修复指数表明受损细胞正常消除。牙周炎患者的积累指数增加可能表明恶性肿瘤的风险。
UNASSIGNED: Periodontitis characterized by mild symptoms in the early stages, which makes diagnostics problematic. The gingival epithelium can be used for micronucleus assay since gums are the area affected by the disease.
UNASSIGNED: The aim of the study was to study the frequency of occurrence and the range of nuclear anomalies in gingival epithelium of healthy people and people with periodontitis.
UNASSIGNED: Scrapings of the gingival epithelium were made next to the central incisors (1.1) and molar teeth (1.7) in control and experimental groups (ten healthy males 35-50 years old and 10 males with periodontitis).
UNASSIGNED: The preparations were stained by Romanowsky-Giemsa. The frequency of nuclear aberrations (‰), the accumulation index, and the repair index were determined.
UNASSIGNED: The differences in the medians of nuclear aberrations were determined using Wilcoxon and the Van-der-Waerden tests. The pathology proportions were compared using the Z-test. To determine the predictors of periodontitis, receiver operator characteristic analysis was used. For multiple comparisons, the Bonferroni correction was used.
UNASSIGNED: In the experimental group, the range of nuclear aberrations was wider, the ratio of karyolysis in the unaffected area was higher, than that in control; perinuclear vacuoles were fewer and macronuclei were more in the affected area. The frequency of cells with micronuclei over 1.33‰ in the affected area is the periodontitis marker.
UNASSIGNED: Gingival epithelium can be used in micronucleus assay. Micronucleus test revealed a wider range of nuclear aberrations in the cells of the gingival epithelium and a higher frequency of occurrence of micronuclei in patients with periodontal disease compared to healthy subjects. Therefore, cytological signs of the inflammation appear earlier than the clinical ones and are verified more clearly. The markers of apoptosis and destruction of nuclei, and low repair index indicate normal elimination of damaged cells. An increased accumulation index in people with periodontitis may indicate the risk of malignant tumors.