■肠易激综合征(IBS)的特征是腹痛和排便习惯的改变。可发酵寡糖,二糖,单糖,和多元醇(FODMAP)是吸收不良的短链碳水化合物,可能会推动共生微生物气体的产生,在IBS中促进腹痛。低FODMAP饮食可导致50%-80%的IBS患者的症状改善。然而,这种饮食并不意味着长期持续,关注下游营养和微生物问题。在这项研究中,我们评估了含有果聚糖水解酶(具有显著菊粉酶活性)的靶向FODMAP酶消化食品补充剂FODMAP酶消化(FODZYME)在模拟胃肠道环境中的功能.
■使用SHIME(人类肠道微生物生态系统模拟器),人体肠道的多隔间模拟器,在模拟的胃肠道条件下的FODZYME剂量发现测定评估了水解3g菊粉的酶能力。全肠模型评估菊粉的消化,果糖的吸收,天然气生产,使用1.125gFODZYME完成了共生微生物行为的其他测量。
■30分钟后,90%的菊粉被1.125g的FODZYME转化为果糖。加倍剂量显示转化率没有显著改善,而半剂量降低性能至77.2%。70%的果糖释放在模拟小肠运输过程中被吸收,随着微生物气体产量的相应减少,丁酸盐和短链脂肪酸产量略有下降。
■FODZYME在有代表性的胃肠道疾病中特别分解菊粉,导致减少的气体产量,同时基本上保留了模型结肠中的短链脂肪酸和丁酸盐的产量。我们的结果表明,膳食补充FODZYME将减少肠道FODMAP负担和气体产生。
UNASSIGNED: Irritable bowel syndrome (
IBS) is characterized by abdominal pain and changes in bowel habits. Fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) are poorly absorbed short-chain carbohydrates that may drive commensal microbial gas production, promoting abdominal pain in
IBS. Low-FODMAP diet can result in symptomatic improvement in 50%-80% of
IBS patients. However, this diet is not meant to be sustained long term, with concern for downstream nutrition and microbial issues. In this study, we evaluate the function of a targeted FODMAP enzymatic digestion food supplement FODMAP enzymatic digestion (FODZYME) containing a fructan-hydrolase enzyme (with significant inulinase activity) in a simulated gastrointestinal environment.
UNASSIGNED: Using SHIME (Simulator of the Human Intestinal Microbial Ecosystem), a multi-compartment simulator of the human gut, FODZYME dose finding assay in modeled gastrointestinal conditions assessed enzymatic ability to hydrolyze 3 g of inulin. Full intestinal modeling assessing digestion of inulin, absorption of fructose, gas production, and other measures of commensal microbial behavior was completed using 1.125 g of FODZYME.
UNASSIGNED: After 30 minutes, 90% of the inulin was converted to fructose by 1.125 g of FODZYME. Doubling dosage showed no significant improvement in conversion, whereas a half dose decreased performance to 77.2%. Seventy percent of released fructose was absorbed during simulated small intestinal transit, with a corresponding decrease in microbial gas production, and a small decrease in butyrate and short-chain fatty acid production.
UNASSIGNED: FODZYME specifically breaks down inulin in representative gastrointestinal conditions, resulting in decreased gas production while substantially preserving short-chain fatty acid and butyrate production in the model colon. Our results suggest dietary supplementation with FODZYME would decrease intestinal FODMAP burden and gas production.