fusobacterium

梭杆菌
  • 文章类型: Journal Article
    尽管幽门螺杆菌是胃癌(GC)中最重要的细菌致癌物,即使在根除幽门螺杆菌后,GC也会出现。研究表明,胃微生物组的各种成分可能会影响GC的发展,但个别病原体的作用尚不清楚。
    通过16SrRNA测序分析人胃粘膜样本,以研究微生物组组成及其与临床参数的关联,包括GC风险。将胃中鉴定的细菌与胃上皮细胞共培养或接种到小鼠体内,和转录组变化,DNA损伤,并对炎症进行了分析。GC组织中的细菌读数与癌症基因组图谱数据集中的转录组和遗传测序数据一起检查。
    根除幽门螺杆菌后的患者根据16SrRNA测序揭示的微生物组成形成了3个亚组。富含梭杆菌属和奈瑟菌属的一个生态失调组与GC发病率明显较高有关。这些物种激活了胃上皮细胞中的原癌基因途径,并促进了小鼠胃中的炎症。构成胃粘蛋白的糖链减弱宿主细菌相互作用。来自梭杆菌属物种的代谢物具有基因毒性,细菌的存在与炎症特征和较高的肿瘤突变负荷相关.
    失调胃中的胃微生物群与根除幽门螺杆菌后的GC发展有关,并通过直接的宿主细菌相互作用发挥致病作用。
    UNASSIGNED: Although Helicobacter pylori is the most important bacterial carcinogen in gastric cancer (GC), GC can emerge even after H. pylori eradication. Studies suggest that various constituents of the gastric microbiome may influence GC development, but the role of individual pathogens is unclear.
    UNASSIGNED: Human gastric mucosal samples were analyzed by 16SrRNA sequencing to investigate microbiome composition and its association with clinical parameters, including GC risk. Identified bacteria in the stomach were cocultured with gastric epithelial cells or inoculated into mice, and transcriptomic changes, DNA damage, and inflammation were analyzed. Bacterial reads in GC tissues were examined together with transcriptomic and genetic sequencing data in the cancer genome atlas dataset.
    UNASSIGNED: Patients after Helicobacter pylori eradication formed 3 subgroups based on the microbial composition revealed by 16SrRNA sequencing. One dysbiotic group enriched with Fusobacterium and Neisseria species was associated with a significantly higher GC incidence. These species activated prooncogenic pathways in gastric epithelial cells and promoted inflammation in mouse stomachs. Sugar chains that constitute gastric mucin attenuate host-bacteria interactions. Metabolites from Fusobacterium species were genotoxic, and the presence of the bacteria was associated with an inflammatory signature and a higher tumor mutation burden.
    UNASSIGNED: Gastric microbiota in the dysbiotic stomach is associated with GC development after H. pylori eradication and plays a pathogenic role through direct host-bacteria interaction.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    背景和目的:代谢功能障碍相关脂肪性肝炎(MASH)相关肝细胞癌(HCC)的发病率在全球范围内呈上升趋势。伴随着肥胖和代谢综合征的流行。根据关于肝癌和牙周炎的潜在关联的初步报告,本研究旨在分析MASH相关HCC(MASH-HCC)中牙周细菌的参与以及口腔和肠道细菌菌群。材料和方法:41例MASH患者和19例MASH-HCC患者参与了研究,完成调查问卷,正在进行牙周检查,提供唾液样本,漱口水,粪便,和外周血。通过16S核糖体RNA测序分析口腔和粪便微生物组谱。采用贝叶斯网络分析法分析各因素之间的因果关系,包括MASH-HCC,考试,和细菌。结果:与MASH组相比,MASH-HCC组的肠道微生物区系中梭杆菌属的占有率明显更高(p=0.002)。然而,Butyricicocus(p=0.022)和Roseburia(p<0.05)的占有率显着降低。贝叶斯网络分析显示,不存在影响HCC的牙周致病菌和肠道细菌。然而,HCC直接影响牙周细菌牙龈卟啉单胞菌,连翘坦菌,具核梭杆菌,和唾液中中中膜普雷沃氏菌,以及乳杆菌属,罗斯布里亚,梭杆菌,普雷沃氏菌,梭菌属,Ruminococus,锥虫,和肠道中的SMB53。此外,口腔中的牙龈卟啉单胞菌直接影响肠道中的乳杆菌属和链球菌属。结论:MASH-HCC直接影响牙周致病菌和肠道细菌,牙龈卟啉单胞菌可能影响与胃肠道癌症相关的肠道细菌。
    Background and Objectives: The incidence of metabolic dysfunction-associated steatohepatitis (MASH)-related hepatocellular carcinoma (HCC) is increasing worldwide, alongside the epidemic of obesity and metabolic syndrome. Based on preliminary reports regarding the potential association of HCC and periodontitis, this study aimed to analyze the involvement of periodontal bacteria as well as the oral and intestinal bacterial flora in MASH-related HCC (MASH-HCC). Materials and Methods: Forty-one patients with MASH and nineteen with MASH-HCC participated in the study, completing survey questionnaires, undergoing periodontal examinations, and providing samples of saliva, mouth-rinsed water, feces, and peripheral blood. The oral and fecal microbiome profiles were analyzed by 16S ribosomal RNA sequencing. Bayesian network analysis was used to analyze the causation between various factors, including MASH-HCC, examinations, and bacteria. Results: The genus Fusobacterium had a significantly higher occupancy rate (p = 0.002) in the intestinal microflora of the MASH-HCC group compared to the MASH group. However, Butyricicoccus (p = 0.022) and Roseburia (p < 0.05) had significantly lower occupancy rates. The Bayesian network analysis revealed the absence of periodontal pathogenic bacteria and enteric bacteria affecting HCC. However, HCC directly affected the periodontal bacterial species Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum, and Prevotella intermedia in the saliva, as well as the genera Lactobacillus, Roseburia, Fusobacterium, Prevotella, Clostridium, Ruminococcus, Trabulsiella, and SMB53 in the intestine. Furthermore, P. gingivalis in the oral cavity directly affected the genera Lactobacillus and Streptococcus in the intestine. Conclusions: MASH-HCC directly affects periodontal pathogenic and intestinal bacteria, and P. gingivalis may affect the intestinal bacteria associated with gastrointestinal cancer.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    目的:本研究旨在评估急性咽炎的病因,并根据病因确定重度和中度症状的持续时间。
    方法:前瞻性观察性研究。网站:一个城市卫生保健中心。
    方法:纳入年龄在15岁或以上的急性咽炎患者。
    方法:在微生物实验室中使用MALDI-TOF对两个咽喉样本进行细菌鉴定。患者接受症状日记一周后返回。
    方法:出现严重症状的天数,在症状日记中包含的任何症状中评分5分或更多,和中度症状,得分3或以上。
    结果:在招募的149名患者中,β溶血性链球菌A组(GABHS)是最常见的病因。仅症状和体征以及平均Centor评分不能区分急性咽炎患者的GABHS和其他细菌原因。然而,有一种趋势表明,由无乳链球菌和无乳链球菌引起的感染表现出更严重的症状,而归因于链球菌群的感染,梭杆菌属。,而那些口咽微生物群被分离的患者症状往往较轻。S.dyproactiactiae感染显示出更长的严重和中度症状持续时间的趋势。
    结论:GABHS是最普遍的,但C组链球菌引起更严重和延长的症状。
    OBJECTIVE: This study aimed to assess the cause of acute pharyngitis and determine the duration of severe and moderate symptoms based on the aetiology.
    METHODS: Prospective observational study. SITE: One urban health care centre.
    METHODS: Patients aged 15 or older with acute pharyngitis were included.
    METHODS: Bacterial identification was carried out in the microbiology lab using MALDI-TOF in two throat samples. Patients received a symptom diary to return after one week.
    METHODS: Number of days with severe symptoms, scoring 5 or more in any of the symptoms included in the symptom diary, and moderate symptoms, scoring 3 or more.
    RESULTS: Among the 149 patients recruited, beta-haemolytic streptococcus group A (GABHS) was the most common aetiology. Symptoms and signs alone as well as the mean Centor score cannot distinguish between GABHS and other bacterial causes in patients with acute pharyngitis. However, there was a trend indicating that infections caused by Streptococcus dysgalactiae and Streptococcus agalactiae presented more severe symptoms, whereas infections attributed to the Streptococcus anginosus group, Fusobacterium spp., and those where oropharyngeal microbiota was isolated tended to have milder symptoms. S. dysgalactiae infections showed a trend towards longer severe and moderate symptom duration.
    CONCLUSIONS: GABHS was the most prevalent, but group C streptococcus caused more severe and prolonged symptoms.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    微生物群落组成与宿主疾病的发生和进展密切相关,强调在各种健康环境中了解宿主微生物群动态的重要性。
    在这项研究中,我们利用全长16SrRNA基因测序对患有口腔恶性纤维瘤的大熊猫(Ailuropodamelanoleuca)口腔中的微生物进行物种级鉴定。
    我们观察到大熊猫口腔肿瘤侧和非肿瘤侧的微生物群落之间存在显着差异,后者表现出更高的微生物多样性。肿瘤侧以特定的微生物为主,比如镰刀菌,卟啉单胞菌sp.猫科动物口服分类单元110,弯曲杆菌。猫科动物口服分类单元100和奈瑟氏菌。猫口服分类单元078,据报道与其他生物体的致瘤过程和牙周疾病相关。根据线性判别分析效应大小分析,在肿瘤侧样本和非肿瘤侧样本之间获得了超过9种不同的生物标志物.此外,《京都基因和基因组百科全书》分析显示,大熊猫口腔微生物群与遗传信息处理和代谢显著相关,特别是辅因子和维生素,氨基酸,和碳水化合物代谢。此外,预计在肿瘤侧有明显的细菌侵袭上皮细胞。
    这项研究为大熊猫口腔微生物群与口腔肿瘤之间的关系提供了重要的见解,并提供了潜在的生物标志物,可以指导圈养和衰老大熊猫的未来健康评估和预防策略。
    UNASSIGNED: Microbial community composition is closely associated with host disease onset and progression, underscoring the importance of understanding host-microbiota dynamics in various health contexts.
    UNASSIGNED: In this study, we utilized full-length 16S rRNA gene sequencing to conduct species-level identification of the microorganisms in the oral cavity of a giant panda (Ailuropoda melanoleuca) with oral malignant fibroma.
    UNASSIGNED: We observed a significant difference between the microbial community of the tumor side and non-tumor side of the oral cavity of the giant panda, with the latter exhibiting higher microbial diversity. The tumor side was dominated by specific microorganisms, such as Fusobacterium simiae, Porphyromonas sp. feline oral taxon 110, Campylobacter sp. feline oral taxon 100, and Neisseria sp. feline oral taxon 078, that have been reported to be associated with tumorigenic processes and periodontal diseases in other organisms. According to the linear discriminant analysis effect size analysis, more than 9 distinct biomarkers were obtained between the tumor side and non-tumor side samples. Furthermore, the Kyoto Encyclopedia of Genes and Genomes analysis revealed that the oral microbiota of the giant panda was significantly associated with genetic information processing and metabolism, particularly cofactor and vitamin, amino acid, and carbohydrate metabolism. Furthermore, a significant bacterial invasion of epithelial cells was predicted in the tumor side.
    UNASSIGNED: This study provides crucial insights into the association between oral microbiota and oral tumors in giant pandas and offers potential biomarkers that may guide future health assessments and preventive strategies for captive and aging giant pandas.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Research Support, Non-U.S. Gov\'t
    暂无摘要。
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    背景:癌症微生物群被认为是癌症进展的主要危险因素。我们已经证明了牙周梭杆菌(F.p)在食管癌(EC)组织中丰度较高。生物信息分析发现,BCT是F.p.的关键毒力蛋白,对BCT在EC中的作用和机制知之甚少。本研究旨在识别F.p的关键毒力蛋白,探讨BCT促进EC的作用机制。
    方法:构建真核表达载体,纯化重组蛋白BCT。CCK8用于分析不同浓度BCT处理后EC的活性。UPLC-MS/MS和ELISA检测代谢组学和代谢产物。通过transwell测定完成迁移和侵袭能力。RT-QPCR,WB用于分析相关基因的表达。
    结果:我们的数据表明,BCT在EC肿瘤组织中的表达更高(p<0.05),BCT在20µg/mL中促进了生存率,EC细胞的侵袭和迁移(EC109)(p<0.05)。同时,UPLC-MS/MS结果表明,BCT导致了低牛磺酸代谢的增强,花生四烯酸代谢,糖酵解/糖异生,色氨酸代谢,EC109中的柠檬酸循环活性。代谢变化导致葡萄糖和丙酮酸水平降低,但乳酸脱氢酶(LDH)活性和乳酸(LA)活性增加以及调节糖酵解的葡萄糖转运蛋白1,己糖激酶2,LDH的表达均发生变化(p<0.05)。BCT处理上调TLR4、Akt、HIF-1α(p<0.05)调节LA的产生。此外,LA刺激促进GPR81、Wnt、和β-连环蛋白(p<0.05),从而在EC109细胞中诱导EMT和转移。
    结论:总而言之,这些发现确定了BCT在调节EC109糖酵解中的影响,其涉及TLR4/Akt/HIF-1α途径。同时,糖酵解通过GPR81/Wnt/β-catenin信号通路增加LA的释放并促进EC109的EMT。总之,我们的研究结果强调了靶向BCT作为缓解EC发展的创新策略的潜力.
    BACKGROUND: The cancer microbiota was considered the main risk factor for cancer progression. We had proved that Fusobacterium periodonticum (F.p) was higher abundance in Esophageal cancer(EC)tissues. Bioinformation analysis found that BCT was a key virulence protein of F.p. However, little is known about the role and mechanism of BCT in EC. This study aimed to recognize the key virulence protein of F.p and explore the mechanism of BCT in promoting EC.
    METHODS: We constructed a eukaryotic expression vector and purified the recombinant protein BCT. CCK8 used to analyzed the activity of EC after treated by different concentration of BCT. UPLC-MS/MS and ELISA used to detect the metabonomics and metabolites. The ability of migration and invasion was completed by transwell assay. RT-QPCR, WB used to analyze the expression of relevant genes.
    RESULTS: Our data showed that BCT was higher expression in EC tumor tissues (p < 0.05) and BCT in 20 µg/mL promoted the survival, invasion and migration of EC cells (EC109) (p < 0.05). Meanwhile, UPLC-MS/MS results suggested that BCT resulted in an augmentation of hypotaurine metabolism, arachidonic acid metabolism, glycolysis/gluconeogenesis, tryptophan metabolism, citrate cycle activity in EC109. The metabolic changes resulted in decreasing in glucose and pyruvate levels but increase in lactate dehydrogenase (LDH) activity and lactic acid (LA) as well as the expression of glucose transporter 1, Hexokinase 2, LDH which regulated the glycolysis were all changed (p < 0.05). The BCT treatment upregulated the expression of TLR4, Akt, HIF-1α (p < 0.05) which regulated the production of LA. Furthermore, LA stimulation promoted the expression of GPR81, Wnt, and β-catenin (p < 0.05), thereby inducing EMT and metastasis in EC109 cells.
    CONCLUSIONS: Altogether, these findings identified that impact of BCT in regulation of glycolysis in EC109 and its involves the TLR4/Akt/HIF-1α pathway. Meanwhile, glycolysis increasing the release of LA and promote the EMT of EC109 by GPR81/Wnt/β-catenin signaling pathway. In summary, our findings underscore the potential of targeting BCT as an innovative strategy to mitigate the development of EC.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Case Reports
    化脓性脊柱感染(PSI)的发生率为每100,000人口0.5-2.2例,尽管诊断可能会延迟长达三个月。有核梭杆菌菌血症的发病率很少见,每10万人发生0.22-0.34例,虽然它在脊柱感染中的影响仍然很少。一名60多岁的患有慢性下背部疼痛的男子被送往急诊科,他有两周的背部疼痛恶化史,伴有发烧和排尿困难。由于最近的尿路感染(UTI)病史,发烧和腰痛,他最初被治疗为肾盂肾炎。然而,右侧有神经根病和双侧髋关节屈曲疼痛,功率降低。右侧和远端L4皮刀的轻触感觉降低。这些与感染标记物紊乱相关的神经功能缺损使椎间盘炎的诊断变得合理。在报告近期发作或严重程度增加的发烧和背痛的患者中,应怀疑有关节炎和天然椎体骨髓炎(NVO)。一旦确认了门徒,随后使用T-SPOT对患者进行了结核病(TB)测试,人类免疫缺陷病毒(HIV),乙型肝炎病毒,和丙型肝炎病毒,没有积极的发现,但在接下来的日子里,血液培养产生F.核仁。通过了解体内的天然水库,建立F.核仁的来源可以通过头颈部成像和检查胃肠道的恶性或炎症过程来实现。
    Pyogenic spinal infections (PSI) have an incidence of 0.5-2.2 cases per 100,000 population, though diagnosis can be delayed by up to three months. The incidence of Fusobacterium nucleatum bacteremia is rare, occurring in 0.22-0.34 cases per 100,000 population, whilst its implication in spinal infections is rarer still. A man in his 60s with a background of chronic lower back pain presented to the emergency department with a two-week history of worsening back pain associated with fever and difficulty voiding. He was initially managed as pyelonephritis due to the recent history of urinary tract infection (UTI) with fever and flank pain. However, there were radiculopathy and bilateral pain on hip flexion with reduced power on the right side. The light-touch sensation was reduced over the right hallux and distal L4 dermatome. These neurological deficits associated with deranged infective markers made a diagnosis of discitis plausible. Discitis and native vertebral osteomyelitis (NVO) should be suspected in patients reporting a fever and back pain of recent onset or increasing in severity. Once discitis was confirmed, the patient was subsequently tested for tuberculosis (TB) using a T-SPOT, human immunodeficiency virus (HIV), hepatitis B virus, and hepatitis C virus, with no positive findings, but in the days following, blood cultures yielded F. nucleatum. Guided by knowing the natural reservoirs in the body, establishing the source of F. nucleatum could be achieved through head and neck imaging and investigating the gastrointestinal tract for malignant or inflammatory processes.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    核梭杆菌是与牙周炎和口腔外疾病相关的口腔厌氧共生菌,包括结直肠癌.先前的研究表明,与口腔发育不良或口腔肿瘤相关的这种细菌的相对丰度增加。使用直接文化,我们发现,从恶性或潜在恶性口腔粘膜中分离出的梭杆菌属物种中有75%是F.nucleatum亚种。多晶型物。在76F.核仁亚种上使用Panaroo进行全基因组测序和pangenome分析。多晶型基因组。F.核仁亚种。在7363个基因的pangenome中,多态性被证明具有1604个基因的相对较小的核心基因组。基于核心基因组的系统发育分析表明,分离株可以分为三个主要分支,与疾病没有明显的基因型关联。从同一患者的健康和患病部位回收的分离物通常高度相关。可以鉴定属于V型分泌系统(TVSS)的大量粘附素,其菌株之间的库和拷贝数存在重大差异。使用fastGEAR对基因间重组的分析表明,粘附素补体是通过水平基因转移和重组而形成的。TVSS粘附素基因的重组事件不仅在多态性亚种谱系之间常见,而且在F.核仁的不同亚种之间。具有低拷贝数TVSS粘附素编码基因的亚种多态性菌株倾向于对口腔角质形成细胞的粘附力最弱。这项研究强调了F.nucleatum亚种的遗传异质性。多晶型物,并为定义该生物体的毒力提供了新的框架。
    Fusobacterium nucleatum is an anaerobic commensal of the oral cavity associated with periodontitis and extra-oral diseases, including colorectal cancer. Previous studies have shown an increased relative abundance of this bacterium associated with oral dysplasia or within oral tumours. Using direct culture, we found that 75 % of Fusobacterium species isolated from malignant or potentially malignant oral mucosa were F. nucleatum subsp. polymorphum. Whole genome sequencing and pangenome analysis with Panaroo was carried out on 76 F. nucleatum subsp. polymorphum genomes. F. nucleatum subsp. polymorphum was shown to possesses a relatively small core genome of 1604 genes in a pangenome of 7363 genes. Phylogenetic analysis based on the core genome shows the isolates can be separated into three main clades with no obvious genotypic associations with disease. Isolates recovered from healthy and diseased sites in the same patient are generally highly related. A large repertoire of adhesins belonging to the type V secretion system (TVSS) could be identified with major variation in repertoire and copy number between strains. Analysis of intergenic recombination using fastGEAR showed that adhesin complement is shaped by horizontal gene transfer and recombination. Recombination events at TVSS adhesin genes were not only common between lineages of subspecies polymorphum, but also between different subspecies of F. nucleatum. Strains of subspecies polymorphum with low copy numbers of TVSS adhesin encoding genes tended to have the weakest adhesion to oral keratinocytes. This study highlights the genetic heterogeneity of F. nucleatum subsp. polymorphum and provides a new framework for defining virulence in this organism.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Case Reports
    化脓性肝脓肿在北美是一个值得注意的健康问题,其特征是死亡率从2%到12%不等。这种情况通常是多微生物的,在西方国家,以链球菌和大肠杆菌为主要病原。梭杆菌属物种,通常是胃肠道的共生体,生殖器,和口腔菌群,与罕见的扁桃体脓肿和Lemierre综合征有关,包括它的胃肠变种称为静脉炎。
    我们介绍了一个有2周腹胀和疼痛史的免疫功能正常的男性病例。腹部磁共振成像显示多间隔囊性肝肿块和门静脉血栓形成。随后的肝活检证实了核梭杆菌的病因。患者开始静脉注射头孢吡肟和口服甲硝唑抗生素。不幸的是,患者在最终诊断之前死于心脏骤停.
    梭杆菌属物种相关肝脓肿,再加上罕见的Lemierre综合征(静脉炎)的胃肠道变异,会带来巨大的死亡风险。该病例强调了与这些病症相关的罕见性和临床挑战。提高临床医生的认识对于早期诊断和及时干预至关重要。在这种情况下可能会改善结果。
    UNASSIGNED: Pyogenic liver abscess is a noteworthy health concern in North America, characterized by a mortality rate ranging from 2 to 12%. This condition is often polymicrobial, with Streptococcus species and Escherichia coli as the predominant causal pathogens in Western countries. Fusobacterium species, typically commensals of gastrointestinal, genital, and oral flora, have been implicated in the rare formation of tonsillar abscesses and Lemierre syndrome, including its gastrointestinal variant known as pylephlebitis.
    UNASSIGNED: We present the case of an immunocompetent male with a 2-week history of abdominal distention and pain. Abdominal magnetic resonance imaging revealed multiseptated cystic hepatic masses and portal vein thrombosis. A subsequent liver biopsy confirmed Fusobacterium nucleatum etiology. The patient was initiated on intravenous cefepime and oral metronidazole antibiotics. Unfortunately, the patient succumbed to cardiac arrest before a final diagnosis could be established.
    UNASSIGNED: Fusobacterium species-associated liver abscess, coupled with the rare gastrointestinal variant of Lemierre syndrome (pylephlebitis), poses a significant mortality risk. This case underscores the rarity and clinical challenges associated with these conditions. Increased awareness among clinicians is crucial for early diagnosis and prompt intervention, potentially improving outcomes in such cases.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    普遍存在的嗜炎性口腔病理性核梭杆菌(Fn)因其与炎性生态失调性疾病和癌症的强烈关联而被广泛认可。Fn细分为四个亚种,历史上被认为在口腔中功能上是可互换的。为了测试这个假设,我们分析了患者匹配的牙菌斑和牙源性脓肿临床标本,并检查了炎症环境是否选择了特定的Fn亚种.牙菌斑含有更大多样性的梭菌,与FN。多晶型物占主导地位,而牙源性脓肿对大部分未表征的生物体Fn异常偏倚。动物。比较基因组分析揭示了Fn亚种之间的显着基因型差异,这些差异与其首选的生态位相关,并支持将每个亚种作为不同的梭杆菌属物种进行分类重新分配。尽管起源于牙菌斑中的低丰度生物,Fn.动物通常在炎性脓肿环境中胜过其他口腔梭菌,这可以解释它在其他口腔和口外疾病中的流行。
    The ubiquitous inflammophilic oral pathobiont Fusobacterium nucleatum (Fn) is widely recognized for its strong association with inflammatory dysbiotic diseases and cancer. Fn is subdivided into four subspecies, which are historically considered functionally interchangeable in the oral cavity. To test this assumption, we analyzed patient-matched dental plaque and odontogenic abscess clinical specimens and examined whether an inflammatory environment selects for/against particular Fn subspecies. Dental plaque harbored a greater diversity of fusobacteria, with Fn. polymorphum dominating, whereas odontogenic abscesses were exceptionally biased for the largely uncharacterized organism Fn. animalis. Comparative genomic analyses revealed significant genotypic distinctions among Fn subspecies that correlate with their preferred ecological niches and support a taxonomic reassignment of each as a distinct Fusobacterium species. Despite originating as a low-abundance organism in dental plaque, Fn. animalis typically outcompetes other oral fusobacteria within the inflammatory abscess environment, which may explain its prevalence in other oral and extraoral diseases.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

公众号