PDF(Pubmed)
Abstract：
UNASSIGNED: Microbial community composition is closely associated with host disease onset and progression, underscoring the importance of understanding host-microbiota dynamics in various health contexts.
UNASSIGNED: In this study, we utilized full-length 16S rRNA gene sequencing to conduct species-level identification of the microorganisms in the oral cavity of a giant panda (Ailuropoda melanoleuca) with oral malignant fibroma.
UNASSIGNED: We observed a significant difference between the microbial community of the tumor side and non-tumor side of the oral cavity of the giant panda, with the latter exhibiting higher microbial diversity. The tumor side was dominated by specific microorganisms, such as Fusobacterium simiae, Porphyromonas sp. feline oral taxon 110, Campylobacter sp. feline oral taxon 100, and Neisseria sp. feline oral taxon 078, that have been reported to be associated with tumorigenic processes and periodontal diseases in other organisms. According to the linear discriminant analysis effect size analysis, more than 9 distinct biomarkers were obtained between the tumor side and non-tumor side samples. Furthermore, the Kyoto Encyclopedia of Genes and Genomes analysis revealed that the oral microbiota of the giant panda was significantly associated with genetic information processing and metabolism, particularly cofactor and vitamin, amino acid, and carbohydrate metabolism. Furthermore, a significant bacterial invasion of epithelial cells was predicted in the tumor side.
UNASSIGNED: This study provides crucial insights into the association between oral microbiota and oral tumors in giant pandas and offers potential biomarkers that may guide future health assessments and preventive strategies for captive and aging giant pandas.
UNASSIGNED: In this study, we utilized full-length 16S rRNA gene sequencing to conduct species-level identification of the microorganisms in the oral cavity of a giant panda (Ailuropoda melanoleuca) with oral malignant fibroma.
UNASSIGNED: We observed a significant difference between the microbial community of the tumor side and non-tumor side of the oral cavity of the giant panda, with the latter exhibiting higher microbial diversity. The tumor side was dominated by specific microorganisms, such as Fusobacterium simiae, Porphyromonas sp. feline oral taxon 110, Campylobacter sp. feline oral taxon 100, and Neisseria sp. feline oral taxon 078, that have been reported to be associated with tumorigenic processes and periodontal diseases in other organisms. According to the linear discriminant analysis effect size analysis, more than 9 distinct biomarkers were obtained between the tumor side and non-tumor side samples. Furthermore, the Kyoto Encyclopedia of Genes and Genomes analysis revealed that the oral microbiota of the giant panda was significantly associated with genetic information processing and metabolism, particularly cofactor and vitamin, amino acid, and carbohydrate metabolism. Furthermore, a significant bacterial invasion of epithelial cells was predicted in the tumor side.
UNASSIGNED: This study provides crucial insights into the association between oral microbiota and oral tumors in giant pandas and offers potential biomarkers that may guide future health assessments and preventive strategies for captive and aging giant pandas.
摘要：
■微生物群落组成与宿主疾病的发生和进展密切相关，强调在各种健康环境中了解宿主微生物群动态的重要性。
■在这项研究中，我们利用全长16SrRNA基因测序对患有口腔恶性纤维瘤的大熊猫（Ailuropodamelanoleuca）口腔中的微生物进行物种级鉴定。
■我们观察到大熊猫口腔肿瘤侧和非肿瘤侧的微生物群落之间存在显着差异，后者表现出更高的微生物多样性。肿瘤侧以特定的微生物为主，比如镰刀菌，卟啉单胞菌sp.猫科动物口服分类单元110，弯曲杆菌。猫科动物口服分类单元100和奈瑟氏菌。猫口服分类单元078，据报道与其他生物体的致瘤过程和牙周疾病相关。根据线性判别分析效应大小分析,在肿瘤侧样本和非肿瘤侧样本之间获得了超过9种不同的生物标志物.此外，《京都基因和基因组百科全书》分析显示，大熊猫口腔微生物群与遗传信息处理和代谢显著相关，特别是辅因子和维生素，氨基酸,和碳水化合物代谢。此外，预计在肿瘤侧有明显的细菌侵袭上皮细胞。
这项研究为大熊猫口腔微生物群与口腔肿瘤之间的关系提供了重要的见解，并提供了潜在的生物标志物，可以指导圈养和衰老大熊猫的未来健康评估和预防策略。
■在这项研究中，我们利用全长16SrRNA基因测序对患有口腔恶性纤维瘤的大熊猫（Ailuropodamelanoleuca）口腔中的微生物进行物种级鉴定。
■我们观察到大熊猫口腔肿瘤侧和非肿瘤侧的微生物群落之间存在显着差异，后者表现出更高的微生物多样性。肿瘤侧以特定的微生物为主，比如镰刀菌，卟啉单胞菌sp.猫科动物口服分类单元110，弯曲杆菌。猫科动物口服分类单元100和奈瑟氏菌。猫口服分类单元078，据报道与其他生物体的致瘤过程和牙周疾病相关。根据线性判别分析效应大小分析,在肿瘤侧样本和非肿瘤侧样本之间获得了超过9种不同的生物标志物.此外，《京都基因和基因组百科全书》分析显示，大熊猫口腔微生物群与遗传信息处理和代谢显著相关，特别是辅因子和维生素，氨基酸,和碳水化合物代谢。此外，预计在肿瘤侧有明显的细菌侵袭上皮细胞。
这项研究为大熊猫口腔微生物群与口腔肿瘤之间的关系提供了重要的见解，并提供了潜在的生物标志物，可以指导圈养和衰老大熊猫的未来健康评估和预防策略。
