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Abstract：
Background and Objectives: The incidence of metabolic dysfunction-associated steatohepatitis (MASH)-related hepatocellular carcinoma (HCC) is increasing worldwide, alongside the epidemic of obesity and metabolic syndrome. Based on preliminary reports regarding the potential association of HCC and periodontitis, this study aimed to analyze the involvement of periodontal bacteria as well as the oral and intestinal bacterial flora in MASH-related HCC (MASH-HCC). Materials and Methods: Forty-one patients with MASH and nineteen with MASH-HCC participated in the study, completing survey questionnaires, undergoing periodontal examinations, and providing samples of saliva, mouth-rinsed water, feces, and peripheral blood. The oral and fecal microbiome profiles were analyzed by 16S ribosomal RNA sequencing. Bayesian network analysis was used to analyze the causation between various factors, including MASH-HCC, examinations, and bacteria. Results: The genus Fusobacterium had a significantly higher occupancy rate (p = 0.002) in the intestinal microflora of the MASH-HCC group compared to the MASH group. However, Butyricicoccus (p = 0.022) and Roseburia (p < 0.05) had significantly lower occupancy rates. The Bayesian network analysis revealed the absence of periodontal pathogenic bacteria and enteric bacteria affecting HCC. However, HCC directly affected the periodontal bacterial species Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum, and Prevotella intermedia in the saliva, as well as the genera Lactobacillus, Roseburia, Fusobacterium, Prevotella, Clostridium, Ruminococcus, Trabulsiella, and SMB53 in the intestine. Furthermore, P. gingivalis in the oral cavity directly affected the genera Lactobacillus and Streptococcus in the intestine. Conclusions: MASH-HCC directly affects periodontal pathogenic and intestinal bacteria, and P. gingivalis may affect the intestinal bacteria associated with gastrointestinal cancer.
摘要：
背景和目的：代谢功能障碍相关脂肪性肝炎（MASH）相关肝细胞癌（HCC）的发病率在全球范围内呈上升趋势。伴随着肥胖和代谢综合征的流行。根据关于肝癌和牙周炎的潜在关联的初步报告，本研究旨在分析MASH相关HCC（MASH-HCC）中牙周细菌的参与以及口腔和肠道细菌菌群。材料和方法：41例MASH患者和19例MASH-HCC患者参与了研究，完成调查问卷，正在进行牙周检查，提供唾液样本，漱口水,粪便,和外周血。通过16S核糖体RNA测序分析口腔和粪便微生物组谱。采用贝叶斯网络分析法分析各因素之间的因果关系,包括MASH-HCC，考试,和细菌。结果：与MASH组相比，MASH-HCC组的肠道微生物区系中梭杆菌属的占有率明显更高（p=0.002）。然而,Butyricicocus（p=0.022）和Roseburia（p<0.05）的占有率显着降低。贝叶斯网络分析显示，不存在影响HCC的牙周致病菌和肠道细菌。然而,HCC直接影响牙周细菌牙龈卟啉单胞菌，连翘坦菌,具核梭杆菌,和唾液中中中膜普雷沃氏菌，以及乳杆菌属，罗斯布里亚,梭杆菌,普雷沃氏菌,梭菌属,Ruminococus,锥虫,和肠道中的SMB53。此外，口腔中的牙龈卟啉单胞菌直接影响肠道中的乳杆菌属和链球菌属。结论:MASH-HCC直接影响牙周致病菌和肠道细菌,牙龈卟啉单胞菌可能影响与胃肠道癌症相关的肠道细菌。
