■肺泡包虫病是由多房棘球蚴引起的,一种具有人畜共患意义的寄生虫,具有广泛的中间和最终宿主,寄生虫在多种条件下成功存活。已经进行了大量研究,以使用线粒体(mt)DNA研究寄生虫种群的遗传结构和亲密关系的水平。本研究是为了调查人口结构,遗传变异,以及提交给全球GenBank的各种多眼大肠杆菌分离株的系统发育关系。mt基因的序列(mt-细胞色素c氧化酶(cox1),分析了多房大肠杆菌的mt-NADH脱氢酶(nad1))以达到设定的目标。
■共有275和124个基因序列的mt-cox1和mt-nad1属于多房性大肠杆菌,分别,是从国家生物技术信息中心GenBank检索的。使用MEGA软件将检索的序列与相应的参考序列进行比对。PopArt软件用于建立中位数加入网络,而DnaSp用于计算中性和多样性指数。MrBayes软件用于基于贝叶斯系统发育研究单倍型之间的系统发育关联。
■nad1和cox1基因的大约13和20个独特的单倍型,分别,在本研究中观察到。在这两个mt基因中,多样性指数表明低单倍型(mt-cox1=0.140;mt-nad1=0.374)和核苷酸(mt-cox1=0.00111;mt-nad1=0.00287)多样性。发现所研究的两个基因的种群的Tajima'sD和FuFs值均为负。
■这项研究是一项首次尝试,旨在在全球范围内提供对多房性大肠杆菌种群结构和遗传变异的见解。然而,为了更好地了解多房菌的种群结构和遗传多样性,应该考虑更多的地理位置和全长基因序列的扩增,这可能有助于扩大对多房性大肠杆菌遗传多样性的认识。
UNASSIGNED: Alveolar echinococcosis is caused by Echinococcus multilocularis, a parasite of zoonotic significance with a wide range of intermediate and final hosts, and the parasite survives successfully in diversified conditions. Plentiful studies have been done to study the genetic structure of the population of the parasite and the level of intimate kinship using mitochondrial (mt) DNA. The present study was conducted to investigate the population structure, genetic variation, and phylogenetic relationship of various isolates of E. multiocularis submitted to GenBank worldwide. Sequences of mt genes (mt-cytochrome c oxidase (
cox1), mt-NADH dehydrogenase (nad1)) of E. multilocularis were analyzed to achieve the set goals.
UNASSIGNED: A total of 275 and 124 gene sequences of mt-
cox1 and mt-nad1 belonging to E. multilocularis, respectively, were retrieved from the National Center for Biotechnology Information GenBank. The retrieved sequences were subjected to alignment with respective reference sequences using MEGA software. The PopArt software was used to establish median-joining networks, while DnaSp was used to calculate neutrality and diversity indices. MrBayes software was used to investigate the phylogenetic association between haplotypes based on Bayesian phylogeny.
UNASSIGNED: Approximately 13 and 20 distinctive haplotypes of nad1 and
cox1 genes, respectively, were observed in the present study. In both of the mt genes, diversity indices indicated low haplotype (mt-
cox1 = 0.140; mt-nad1 = 0.374) and nucleotide (mt-
cox1 = 0.00111; mt-nad1 = 0.00287) diversities. The values of Tajima\'s D and Fu Fs for a population of both of the genes under study were found to be negative.
UNASSIGNED: This study is a maiden attempt to provide insights into the population structure and genetic variation of E. multilocularis on a global scale. However, it is suggested that to better understand the population structure and genetic diversity of E. multilocularis, more geographical locations and amplifications of full-length gene sequences should be considered, which could be helpful in widening the insights into the genetic diversity of E. multilocularis.