■微丝虫寄生虫在热带地区很常见,据报道,一些物种可能是人畜共患的。通过细胞学或血液学技术诊断犬丝虫感染的敏感性和特异性较低,这可能导致误诊。因此,分子技术似乎是识别和检测微丝虫感染的替代方法。另一方面,淋巴瘤是家畜的主要肿瘤之一,在家犬中患病率很高。这项研究旨在报道患有淋巴瘤的狗与微丝虫的混合感染,强调其诊断以及这种感染在瘤形成发展中的可能作用。
■由于嗜睡,一只8岁的雄性混种狗被转诊,reumbency,皮肤溃疡病变,非特异性疼痛表现,呕吐,左颞肌的肌阵鸣,和癫痫发作。血常规和生化检查正常,皮肤的细胞学检查证实了微丝虫感染。那只狗死于心肺骤停,尸检时进行组织取样进行组织病理学和分子分析.
■皮肤病变与Acanthocheilonemareconditum相关的微丝虫性肉芽肿有关。脾脏和肝脏的组织病理学显示由母细胞和大淋巴细胞组成的弥漫性淋巴瘤,广泛分布在薄壁组织和脉管系统周围。在皮肤上,在一些浅表毛细血管中可见微丝虫。
■这项研究描述了患有淋巴瘤的狗的微丝虫混合感染。根据作者的知识,这是关于狗肿瘤中混合微丝虫感染的第一份报告,并强调了分子技术的使用,即,聚合酶链反应,为了准确的诊断。
UNASSIGNED: Microfilariae parasites are common in tropical regions, and some species are reported as potentially zoonotic. The diagnosis of filarial infection in dogs by cytology or hematologic techniques showed lower sensibility and specificity, which may result in misdiagnosis. Thus, molecular techniques seem to be an alternative to identifying and detecting microfilariae infections. On the other hand, lymphoma is one of the main tumors in domestic animals, with a high prevalence in domestic canines. This study aims to report a mixed infection with microfilariae in a dog with lymphoma, emphasizing its diagnosis and the possible role of this infection in the development of the neoplasia.
UNASSIGNED: An 8-year-old male mixed breed dog was referred to consultation due to the presence of lethargy, recumbency, skin ulceration lesions, nonspecific pain manifestations, emesis, myoclonus in the left temporalis muscle, and seizures. Routine blood and biochemistry tests were normal, and cytology of the skin evidenced a microfilariae infection. The dog died due to a cardiorespiratory arrest, and tissue sampling was done for histopathology and molecular analysis at the necropsy examination.
UNASSIGNED: Skin lesions were related to a microfilarial pyogranuloma related to Acanthocheilonema reconditum. Histopathology of the spleen and liver revealed a diffuse lymphoma composed of blast cells and large lymphocytes, distributed diffusely in the parenchyma and surrounding the vasculature. In the skin, microfilariae were seen in some superficial capillaries.
UNASSIGNED: This study describes a microfilariae mixed infection with A. reconditum and Dirofilaria immitis in a dog with a lymphoma and its molecular detection. To the knowledge of the authors, this is the first report of a mixed microfilariae infection in a tumor of a dog and highlights the use of molecular techniques, i.e., polymerase chain reaction, for an accurate diagnosis.