背景:肉囊虫是强制性的异氧寄生虫,其中一些是人畜共患的,代表公共卫生和经济影响。本研究调查了结节虫的发生。从比利时屠宰场抽取的牛。
方法:本研究共纳入200具尸体,在10个采样日期间采样。应用沉降法从每个car体收集的心脏和diaphragm肌分离肌囊。多重PCR,PCR-RFLP以及cox1基因测序技术被连续应用于收集的肌囊进行物种鉴定。
结果:肉囊虫。在64%(128/200;95%CI57-71%)的采样尸体中检测到。与雌性牛肉和雄性牛肉相比,雌性奶牛的肉孢子虫发生率最高(91%),并且肉孢子虫的物种多样性最高。结节虫。在女性牛肉(p<0.001)和乳制品尸体(p=0.001)中,在心肌中检测到的频率高于隔膜,而在男性尸体中没有观察到显着差异(p=0.763)。年龄对男性尸体的影响不显著(p=0.872),而在两种类型的雌性尸体中,发现肌囊的几率随着年龄的增长而显着增加(p=0.003)。克鲁兹是最普遍的物种,在56.5%(113/200)的尸体中发现,其次是人源链球菌(21.0%,42/200),S、bovifelis(12.5%,25/200),S、博维尼(2.0%,4/200),S、hirsuta(1.5%,3/200)和S.heydorni(0.5%,1/200)。在隔膜中检测到六种不同的物种,而只有两个物种从心脏中被回收。克鲁兹是心脏中最常见的物种,在隔膜中,这是S.hominis.
结论:在21%的尸体样本中检测到人源链球菌存在潜在的食品安全问题,并且需要进一步的研究来控制这种感染。
BACKGROUND: Sarcocystis species are obligatorily heteroxenous parasites, of which some are zoonotic, representing a public health and economic impact. This
study investigated the occurrence of Sarcocystis spp. in cattle sampled from a Belgian slaughterhouse.
METHODS: A total of 200 carcasses were included in the
study, sampled during 10 sampling days. The sedimentation method was applied to isolate the sarcocysts from both heart and diaphragm muscles collected from each carcass. Multiplex PCR, PCR-RFLP as well as
cox1 gene sequencing techniques were applied serially on collected sarcocysts for species identification.
RESULTS: Sarcocystis spp. were detected in 64% (128/200; 95% CI 57-71%) of the sampled carcasses. Female dairy cattle presented the highest Sarcocystis occurrence rate (91%) as well as the highest Sarcocystis species diversity compared to female beef and male beef. Sarcocystis spp. were detected more often in the heart muscles than in the diaphragm among female beef (p < 0.001) and dairy carcasses (p = 0.001), while in male carcasses no significant difference was observed (p = 0.763). The effect of age was not significant in male carcasses (p = 0.872), while the odds of finding sarcocysts significantly increased with age (p = 0.003) within both types of female carcasses. S. cruzi was the most prevalent species and was found in 56.5% (113/200) of the carcasses, followed by S. hominis (21.0%, 42/200), S. bovifelis (12.5%, 25/200), S. bovini (2.0%, 4/200), S. hirsuta (1.5%, 3/200) and S. heydorni (0.5%, 1/200). Six different species were detected in the diaphragm, while only two species were recovered from the heart. S. cruzi was the most prevalent species in heart, while in the diaphragm, this was S. hominis.
CONCLUSIONS: The detection of S. hominis in 21% of the sampled carcasses presents a potential food safety issue, and further research is warranted into controlling this infection.