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Abstract：
Research on complete mitochondrial genomes can help in understanding the molecular evolution and phylogenetic relationships of various species. In this study, the complete mitogenome of Huaaristarchorum was characterized to supplement the limited mitogenomic information on the genus Hua. Three distinct assembly methods, GetOrganelle, NovoPlasty and SPAdes, were used to ensure reliable assembly. The 15,691 bp mitogenome contains 37 genes and an AT-rich region. Notably, the cytochrome c oxidase subunit I (COX1) gene, commonly used for species identification, appears to be slow-evolving and less variable, which may suggest the inclusion of rapidly evolving genes (NADH dehydrogenase subunit 6 [ND6] or NADH dehydrogenase subunit 2 [ND2]) as markers in diagnostic, detection, and population genetic studies of Cerithioidea. Moreover, we identified the unreliability of annotations (e.g., the absence of annotations for NADH dehydrogenase subunit 4L [ND4L] in NC_037771) and potential misidentifications (NC_023364) in public databases, which indicate that data from public databases should be manually curated in future research. Phylogenetic analyses of Cerithioidea based on different datasets generated identical trees using maximum likelihood and Bayesian inference methods. The results confirm that Semisulcospiridae is closely related to Pleuroceridae. The sequences of Semisulcospiridae clustered into three clades, of which H.aristarchorum is one; H.aristarchorum is sister to the other two clades. The findings of this study will contribute to a better understanding of the characteristics of the H.aristarchorum mitogenome and the phylogenetic relationships of Semisulcospiridae. The inclusion of further mitochondrial genome sequences will improve knowledge of the phylogeny and origin of Cerithioidea.
摘要：
对完整线粒体基因组的研究有助于理解各种物种的分子进化和系统发育关系。在这项研究中,华氏完整的有丝分裂基因组的特征是补充华氏属有限的有丝分裂基因组信息。三种不同的组装方法，GetOrganelle,NovoPlasty和SPAdes，用于确保可靠的组装。15,691bp的有丝分裂基因组包含37个基因和一个富含AT的区域。值得注意的是,细胞色素c氧化酶亚基I（COX1）基因，通常用于物种鉴定，看起来进化缓慢，变量较少，这可能表明包含快速进化的基因（NADH脱氢酶亚基6[ND6]或NADH脱氢酶亚基2[ND2]）作为诊断标记，检测,和Cerithiidea的种群遗传研究。此外，我们确定了注释的不可靠性(例如，NC_037771中NADH脱氢酶亚基4L[ND4L]的注释缺失）和公共数据库中的潜在错误识别（NC_023364），这表明来自公共数据库的数据应该在未来的研究中手动整理。基于不同数据集的Cerithioidea的系统发育分析使用最大似然和贝叶斯推断方法生成了相同的树。结果证实半螺旋体科与胸膜科密切相关。半螺旋体科的序列分为三个分支，其中H.aristarchorum是其中之一；H.aristarchorum是其他两个分支的姐妹。这项研究的发现将有助于更好地理解H.aristarchorum有丝分裂基因组的特征和半sulcospiridae的系统发育关系。包含更多的线粒体基因组序列将改善对Cerithioidea的系统发育和起源的了解。
