卫星DNA重复序列是在真核基因组中发现的重复DNA序列,通常由串联阵列中重复的短DNA基序组成。尽管有大量关于其他分类群卫星DNA重复的文献,专门针对Tettigoniidae的调查仍然明显缺乏。我们的研究旨在填补我们对形成Tettigoniidae基因组的卫星进化过程的理解的关键空白。重复体分析显示,地中海地中海的基因组占92%,白灵芝的最低值为34%,在其他Tettigoniidae物种中平均占67%。分析揭示了Tettigoniidae家族物种之间卫星DNA重复序列的显着差异,地中海分枝杆菌数量最高,246,迄今为止在昆虫中报告,数量最低,10,在灰翅目中。鲁斯波利亚·杜比亚和鲁斯波利亚·云南,是同属物种,展示104个和84个家庭的不同数量,分别。R.dubia中的卫星DNA重复表现出最高的丰度,占总基因组的17.2%,虽然在灰翅目中报道的丰度最低,5.65%。基因组大小与卫星DNA家族计数弱相关(rs=0.42,p=0.29),但是卫星丰度与家庭数量之间存在很强的相关性(rs=0.73,p=0.03)。此外,卫星DNA增益和损失模式的分析提供了对基因组中卫星DNA家族的扩增和均质化的见解,物种特异性重复序列表现出积极的扩增趋势。地中海分枝杆菌的染色体分布表明,在Chr12,Chr01和Chr04上观察到最高的积累,占17.79%,17.4%,占总染色体大小的17.22%,分别。卫星DNA家族的染色体特异性繁殖很明显,MthSat01仅在1号染色体上,MthSat170在2号染色体上,共享1.64%和2.33%。观察到的卫星DNA数量和丰度的保守性和变化,以及不同的得失模式,表明潜在的不同进化过程对这些昆虫基因组景观的影响,这需要进一步调查。此外,卫星DNA在特定染色体上的差异积累意味着潜在的染色体特异性功能或结构特征会影响卫星序列的保留和增殖。
Satellite DNA repeats are repetitive DNA sequences found in eukaryotic genomes, typically consisting of short DNA motifs repeated in tandem arrays. Despite the vast body of literature on satellite DNA repeats in other taxa, investigations specifically targeting Tettigoniidae remain conspicuously absent. Our study aims to fill a critical gap in our understanding of satellitome evolutionary processes shaping Tettigoniidae genomes. Repeatome analysis revealed that the Meconema thalassinum genome comprises 92%, and Phryganogryllacris superangulata had the lowest value of 34%, with an average of 67% in other Tettigoniidae species. The analysis reveals significant variation in the number of satellite DNA repeats across species of the Tettigoniidae family, with M. thalassinum exhibiting the highest count, 246, reported in insects to date and the lowest count, 10, in Pholidoptera griseoptera. Ruspolia dubia and Ruspolia yunnana, which are congeneric species, showcase distinct counts of 104 and 84 families, respectively. Satellite DNA repeats in R. dubia exhibit the highest abundance, constituting 17.2% of the total genome, while the lowest abundance was reported in P. griseoptera, at 5.65%. The genome size correlates weakly with the satellite DNA family count (rs = 0.42, p = 0.29), but a strong correlation exists between satellite abundance and family number (rs = 0.73, p = 0.03). Moreover, the analysis of satellite DNA gain and loss patterns provides insights into the amplification and homogenization of satellite DNA families within the genome, with species-specific repeats exhibiting a positive trend toward amplification. The chromosomal distribution in M. thalassinum displayed that the highest accumulation was observed on Chr12, Chr01, and Chr04, constituting 17.79%, 17.4%, and 17.22% of the total chromosome size, respectively. The chromosome-specific propagation of satellite DNA families was evident, with MthSat01 solely on chromosome 1 and MthSat170 on chromosome 2, sharing 1.64% and 2.33%. The observed conservation and variations in satellite DNA number and abundances, along with distinct patterns of gain and loss, indicate the influence of potentially diverse evolutionary processes shaping the genomic landscape of these insects, which requires further investigation. Furthermore, the differential accumulation of satellite DNA on specific chromosomes implies that potential chromosome-specific functions or structural features influence the retention and proliferation of satellite sequences.