PDF(Pubmed)
Abstract：
BACKGROUND: Transposable elements (TEs) are major components of eukaryotic genomes. The extensive body of evidence suggests that although they were once considered \"genomic parasites\", transposons and their transcripts perform specific functions, such as regulation of early embryo development. Understanding the role of TEs in such parasites as trematodes is becoming critically important. Fasciola hepatica, a parasite affecting humans and livestock, undergoes a complex life cycle in diverse environments and hosts, and knowledge about its life cycle regulation is scarce so far.
METHODS: We summarized the data regarding the repetitive elements in F. hepatica and conducted bulk RNA-seq analysis across its life cycle stages. TE expression profiles were analyzed, focusing on differential expression and potential homology with previously described long non-coding RNAs (lncRNAs).
RESULTS: Differential expression analysis revealed stage-specific TE transcription patterns, notably peaking during egg and metacercariae stages. Some TEs showed homology with known lncRNAs and contained putative transcription factor binding sites. Interestingly, TE transcription levels were highest in eggs and metacercariae compared to adults, suggesting regulatory roles in trematode life cycle transitions.
CONCLUSIONS: These findings suggest that TEs may play roles in regulating trematode life cycle transitions. Moreover, TE homology with lncRNAs underscores their significance in gene regulation.
METHODS: We summarized the data regarding the repetitive elements in F. hepatica and conducted bulk RNA-seq analysis across its life cycle stages. TE expression profiles were analyzed, focusing on differential expression and potential homology with previously described long non-coding RNAs (lncRNAs).
RESULTS: Differential expression analysis revealed stage-specific TE transcription patterns, notably peaking during egg and metacercariae stages. Some TEs showed homology with known lncRNAs and contained putative transcription factor binding sites. Interestingly, TE transcription levels were highest in eggs and metacercariae compared to adults, suggesting regulatory roles in trematode life cycle transitions.
CONCLUSIONS: These findings suggest that TEs may play roles in regulating trematode life cycle transitions. Moreover, TE homology with lncRNAs underscores their significance in gene regulation.
摘要：
背景：转座因子（TE）是真核生物基因组的主要组成部分。大量的证据表明，尽管它们曾经被认为是“基因组寄生虫”，转座子及其转录本执行特定功能，如早期胚胎发育的调节。了解TEs在吸虫等寄生虫中的作用变得至关重要。肝片吸虫,一种影响人类和牲畜的寄生虫，在不同的环境和主机中经历复杂的生命周期，到目前为止，关于其生命周期调节的知识还很少。
方法：我们总结了有关肝肝菌中重复元件的数据，并在其生命周期阶段进行了大量RNA-seq分析。TE表达谱进行了分析，关注与先前描述的长链非编码RNA（lncRNAs）的差异表达和潜在同源性。
结果：差异表达分析揭示了阶段特异性TE转录模式，尤其是在卵和囊虫阶段达到峰值。一些TE与已知的lncRNAs具有同源性，并含有推定的转录因子结合位点。有趣的是,与成虫相比，卵和尾虫中的TE转录水平最高，提示吸虫生命周期转变中的调节作用。
结论：这些研究结果表明，TEs可能在调节吸虫生命周期转变中发挥作用。此外，TE与lncRNAs的同源性强调了它们在基因调控中的重要性。
方法：我们总结了有关肝肝菌中重复元件的数据，并在其生命周期阶段进行了大量RNA-seq分析。TE表达谱进行了分析，关注与先前描述的长链非编码RNA（lncRNAs）的差异表达和潜在同源性。
结果：差异表达分析揭示了阶段特异性TE转录模式，尤其是在卵和囊虫阶段达到峰值。一些TE与已知的lncRNAs具有同源性，并含有推定的转录因子结合位点。有趣的是,与成虫相比，卵和尾虫中的TE转录水平最高，提示吸虫生命周期转变中的调节作用。
结论：这些研究结果表明，TEs可能在调节吸虫生命周期转变中发挥作用。此外，TE与lncRNAs的同源性强调了它们在基因调控中的重要性。
