METHODS: We summarized the data regarding the repetitive elements in F. hepatica and conducted bulk RNA-seq analysis across its life cycle stages. TE expression profiles were analyzed, focusing on differential expression and potential homology with previously described long non-coding RNAs (lncRNAs).
RESULTS: Differential expression analysis revealed stage-specific TE transcription patterns, notably peaking during egg and metacercariae stages. Some TEs showed homology with known lncRNAs and contained putative transcription factor binding sites. Interestingly, TE transcription levels were highest in eggs and metacercariae compared to adults, suggesting regulatory roles in trematode life cycle transitions.
CONCLUSIONS: These findings suggest that TEs may play roles in regulating trematode life cycle transitions. Moreover, TE homology with lncRNAs underscores their significance in gene regulation.
方法:我们总结了有关肝肝菌中重复元件的数据,并在其生命周期阶段进行了大量RNA-seq分析。TE表达谱进行了分析,关注与先前描述的长链非编码RNA(lncRNAs)的差异表达和潜在同源性。
结果:差异表达分析揭示了阶段特异性TE转录模式,尤其是在卵和囊虫阶段达到峰值。一些TE与已知的lncRNAs具有同源性,并含有推定的转录因子结合位点。有趣的是,与成虫相比,卵和尾虫中的TE转录水平最高,提示吸虫生命周期转变中的调节作用。
结论:这些研究结果表明,TEs可能在调节吸虫生命周期转变中发挥作用。此外,TE与lncRNAs的同源性强调了它们在基因调控中的重要性。