目的:研究强调了臭氧治疗在医学和牙科领域的许多好处,包括它对各种病原微生物的抗菌功效,它有效调节免疫系统的能力,减少炎症,防止缺氧,并支持组织再生。然而,其对拔牙愈合的影响仍有待阐明。.因此,这项研究旨在评估不同剂量的全身臭氧(O3)在大鼠拔牙槽愈合中的作用。
方法:为此,72只Wistar大鼠拔除右上中切牙后随机分为四组:C组—对照,无全身治疗;OZ0.3组-动物接受0.3mg/kgO3的单剂量;OZ0.7组-0.7mg/kgO3的单剂量;和OZ1.0组-腹膜内接受1.0mg/kgO3的单剂量。总的来说,治疗开始后第7,14和21天,每组6只动物实施安乐死.收集骨样本,并通过描述性组织学进一步分析,组织形态计量学,骨钙蛋白(OCN)和抗酒石酸酸性磷酸酶(TRAP)蛋白表达的免疫组化。
结果:与C组相比,21天后所有应用剂量的O3均显示增加骨组织(PBT)的百分比。14天后,与C组相比,OZ0.7和OZ1.0组显示出明显更高的PBT。OZ1.0组表现出关于PBT的最有益结果。这表示剂量依赖性反应。在第21天,所有组的OCN免疫染色均较高。然而,七天和十四天后,OZ1.0组较C组OCN免疫染色显著增加。在各组和时间点之间没有发现TRAP+破骨细胞的差异。
结论:因此,较高剂量的O3治疗可能有利于拔牙后牙槽骨的修复。
OBJECTIVE: Studies have highlighted numerous benefits of ozone therapy in the field of medicine and dentistry, including its antimicrobial efficacy against various pathogenic microorganisms, its ability to modulate the immune system effectively, reduce inflammation, prevent hypoxia, and support tissue regeneration. However, its effects on dental extraction healing remain to be elucidated. .Therefore, this study aimed to evaluate the effects of systemically administered ozone (O3) at different doses in the healing of dental extraction sockets in rats.
METHODS: To this end, 72 Wistar rats were randomly divided into four groups after extraction of the right upper central incisor: Group C - control, no systemic treatment; Group OZ0.3 - animals received a single dose of 0.3 mg/kg O3; Group OZ0.7 - a single dose of 0.7 mg/kg O3; and Group OZ1.0 - a single dose of 1.0 mg/kg O3, intraperitoneally. In total, six animals from each group were euthanized at 7, 14, and 21 days after the commencement of treatment. Bone samples were harvested and further analyzed by descriptive histology, histomorphometry, and immunohistochemistry for osteocalcin (OCN) and tartrate-resistant acid phosphatase (TRAP) protein expression.
RESULTS: All applied doses of O3 were shown to increase the percentage of bone tissue (PBT) after 21 days compared to group C. After 14 days, the OZ0.7 and OZ1.0 groups showed significantly higher PBT when compared to group C. The OZ1.0 group presented the most beneficial results regarding PBT among groups, which denotes a dose-dependent response. OCN immunostaining was higher in all groups at 21 days. However, after seven and 14 days, the OZ1.0 group showed a significant increase in OCN immunostaining compared to C group. No differences in TRAP+ osteoclasts were found between groups and time points.
CONCLUSIONS: Therefore, O3 therapy at higher doses might be beneficial for bone repair of the alveolar socket following tooth extraction.