PDF(Pubmed)
Abstract：
Orthodontic space closure following tooth extraction is often hindered by alveolar bone deficiency. This study investigates the therapeutic use of nuclear factor-kappa B (NF-κB) decoy oligodeoxynucleotides loaded with polylactic-co-glycolic acid nanospheres (PLGA-NfDs) to mitigate alveolar bone loss during orthodontic tooth movement (OTM) following the bilateral extraction of maxillary first molars in a controlled experiment involving forty rats of OTM model with ethics approved. The decreased tendency of the OTM distance and inclination angle with increased bone volume and improved trabecular bone structure indicated minimized alveolar bone destruction. Reverse transcription-quantitative polymerase chain reaction and histomorphometric analysis demonstrated the suppression of inflammation and bone resorption by downregulating the expression of tartrate-resistant acid phosphatase, tumor necrosis factor-α, interleukin-1β, cathepsin K, NF-κB p65, and receptor activator of NF-κB ligand while provoking periodontal regeneration by upregulating the expression of alkaline phosphatase, transforming growth factor-β1, osteopontin, and fibroblast growth factor-2. Importantly, relative gene expression over the maxillary second molar compression side in proximity to the alveolus highlighted the pharmacological effect of intra-socket PLGA-NfD administration, as evidenced by elevated osteocalcin expression, indicative of enhanced osteocytogenesis. These findings emphasize that locally administered PLGA-NfD serves as an effective inflammatory suppressor and yields periodontal regenerative responses following tooth extraction.
摘要：
拔牙后的正畸间隙闭合通常会因牙槽骨不足而受阻。这项研究调查了装载有聚乳酸-co-乙醇酸纳米球（PLGA-NfDs）的核因子-κB（NF-κB）诱饵寡脱氧核苷酸在双侧拔除上颌第一磨牙后减轻正畸牙齿移动（OTM）过程中牙槽骨丢失的治疗用途。随着骨体积的增加和小梁骨结构的改善，OTM距离和倾斜角的降低趋势表明牙槽骨破坏最小化。逆转录-定量聚合酶链反应和组织形态学分析表明，通过下调抗酒石酸酸性磷酸酶的表达来抑制炎症和骨吸收。肿瘤坏死因子-α，白细胞介素-1β，组织蛋白酶K,NF-κBp65和NF-κB受体活化因子配体通过上调碱性磷酸酶的表达引起牙周再生，转化生长因子-β1，骨桥蛋白，和成纤维细胞生长因子-2。重要的是,上颌第二磨牙靠近牙槽侧的相对基因表达突出了槽内PLGA-NfD给药的药理作用，正如骨钙蛋白表达升高所证明的那样，表明成骨细胞生成增强。这些发现强调了局部给药的PLGA-NfD是一种有效的炎症抑制剂，并在拔牙后产生牙周再生反应。
