猪带虫的人囊虫病是非洲国家神经系统疾病的主要原因,东南亚,和美洲。出版了四个死体基因组(T.solium,多房棘球蚴,E.granulosus和膜状微气孔)在过去的十年中,标志着研究对人类和动物健康具有重要意义的c虫寄生虫的宿主-寄生虫分子串扰的新方法的出现。头肌带虫是另一种寄生虫,与T.solium密切相关,已在许多研究中用作人类囊虫病的动物模型。因此,T.crassiceps基因组的表征也将有助于人类感染的理解。这里,我们报道了T.crassicepsWFU菌株的基因组,重建为不连续的完成分辨率,并针对ORF菌株进行了基因组和差异表达比较分析。使用牛津纳米孔(MinION)和Illumina技术对这两个菌株的基因组进行了测序,为两种菌株实现约107Mb的高质量组装。WFU和ORF之间的点图比较表明,两个基因组非常相似。此外,两种菌株的核型分析结果均未能证明染色体组成存在差异。因此,我们的结果强烈支持这样的观点,即在巨头T.crassiceps的ORF菌株中没有scoleex并不是其他地方提出的染色体丢失的结果.相反,这似乎是基因表达调控微妙而广泛差异的结果。两个菌株之间的变异分析确定了2,487个位点,其变化分布在65个支架中的31个中。差异表达分析显示,与ORF菌株的发育和形态发生有关的基因可能与头型形成的缺乏有关。
Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. Publication of four cestode genomes (T. solium, Echinococcus multilocularis, E. granulosus and Hymenolepis microstoma) in the last decade, marked the advent of novel approaches on the study of the host-parasite molecular crosstalk for cestode parasites of importance for human and animal health. Taenia crassiceps is another cestode parasite, closely related to T. solium, which has been used in numerous studies as an animal model for human cysticercosis. Therefore, characterization of the T. crassiceps genome will also contribute to the understanding of the human infection. Here, we report the genome of T. crassiceps WFU strain, reconstructed to a noncontiguous finished resolution and performed a genomic and differential expression comparison analysis against ORF strain. Both strain genomes were sequenced using Oxford Nanopore (MinION) and Illumina technologies, achieving high quality assemblies of about 107 Mb for both strains. Dotplot comparison between WFU and ORF demonstrated that both genomes were extremely similar. Additionally, karyotyping results for both strains failed to demonstrate a difference in chromosome composition. Therefore, our results strongly support the concept that the absence of scolex in the ORF strain of T. crassiceps was not the result of a chromosomal loss as proposed elsewhere. Instead, it appears to be the result of subtle and extensive differences in the regulation of gene expression. Analysis of variants between the two strains identified 2,487 sites with changes distributed in 31 of 65 scaffolds. The differential expression analysis revealed that genes related to development and morphogenesis in the ORF strain might be involved in the lack of scolex formation.