PDF(Pubmed)
Abstract：
Taenia crassiceps is a zoonotic tapeworm of the genus Taenia that is distributed throughout the Northern Hemisphere. Wild and domestic carnivores are final hosts, while rodents and rabbits are primarily intermediate hosts, although many other mammals may harbour the larval stage, Cysticercus longicollis. This case report aims to describe C. longicollis infection in a lemur and molecularly characterise the isolated parasite. The excised lesion was subjected to morphological and histopathological examination, which revealed cysticerci of the tapeworm. Formalin-fixed and paraffin-embedded block (FFPEB), as well as the cysticerci fixed with formalin stored for one year, were subjected to molecular analysis, which aimed at detecting the partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of Taenia sp. Based on the morphological characteristics, the parasite was identified as a metacestode of T. crassiceps. The presence of the cox1 gene was detected using polymerase chain reaction (PCR) in all samples. A randomly selected PCR product was sequenced and compared with other sequences from the GenBank database, confirming that the detected parasite was T. crassiceps. This article reports the first case of T. crassiceps cysticercosis in a lemur (Lemur catta) in Croatia and emphasises the potential risk of transmission from wild carnivores.
摘要：
牛带虫是牛带虫属的人畜共患tape虫，分布在整个北半球。野生和家养食肉动物是最后的寄主，而啮齿动物和兔子主要是中间宿主，尽管许多其他哺乳动物可能处于幼体阶段，长囊尾囊。本病例报告旨在描述狐猴中的长颈梭菌感染，并对分离的寄生虫进行分子表征。切除的病灶接受形态学和组织病理学检查,这显示出了丝虫的囊尾虫。福尔马林固定和石蜡包埋块（FFPEB），以及用福尔马林固定的囊尾蚴保存了一年，进行了分子分析，旨在检测Taeniasp的部分线粒体细胞色素c氧化酶亚基1（cox1）基因。根据形态特征,该寄生虫被鉴定为T.crassiceps的后继。在所有样品中使用聚合酶链反应(PCR)检测cox1基因的存在。对随机选择的PCR产物进行测序，并与GenBank数据库中的其他序列进行比较，确认检测到的寄生虫是T.crassiceps.本文报道了克罗地亚狐猴（Lemurcatta）中的第一例T.crassiceps囊虫病，并强调了野生食肉动物传播的潜在风险。
