PDF(Pubmed)
Abstract：
Some parasites are known to influence brain proteins or induce changes in the functioning of the nervous system. In this study, our objective is to demonstrate how the two-dimensional gel technique is valuable for detecting differences in protein expression and providing detailed information on changes in the brain proteome during a parasitic infection. Subsequently, we seek to understand how the parasitic infection affects the protein composition in the brain and how this may be related to changes in brain function. By analyzing de novo-expressed proteins at 2, 4, and 8 weeks post-infection compared to the brains of the control mice, we observed that proteins expressed at 2 weeks are primarily associated with neuroprotection or the initial response of the mouse brain to the infection. At 8 weeks, parasitic infection can induce oxidative stress in the brain, potentially activating signaling pathways related to the response to cellular damage. Proteins expressed at 8 weeks exhibit a pattern indicating that, as the host fails to balance the Neuro-Immuno-Endocrine network of the organism, the brain begins to undergo an apoptotic process and consequently experiences brain damage.
摘要：
已知一些寄生虫会影响脑蛋白或引起神经系统功能的变化。在这项研究中,我们的目的是证明二维凝胶技术在检测蛋白质表达差异和提供寄生虫感染期间大脑蛋白质组变化的详细信息方面是有价值的.随后，我们试图了解寄生虫感染如何影响大脑中的蛋白质组成，以及这可能与大脑功能的变化有关。通过分析感染后2、4和8周的从头表达的蛋白质，与对照小鼠的大脑相比，我们观察到2周时表达的蛋白质主要与神经保护或小鼠大脑对感染的初始反应有关。在8周的时候,寄生虫感染可以在大脑中引起氧化应激，潜在激活与细胞损伤反应相关的信号通路。在8周表达的蛋白质表现出一种模式，表明，由于宿主无法平衡生物体的神经-免疫-内分泌网络，大脑开始经历凋亡过程，因此经历脑损伤。
