PDF(Pubmed)
Abstract：
Flatworms are known for their remarkable regenerative ability, one which depends on totipotent cells known as germinative cells in cestodes. Depletion of germinative cells with hydroxyurea (HU) affects the regeneration of the parasite. Here, we studied the reduction and recovery of germinative cells in T. crassiceps cysticerci after HU treatment (25 mM and 40 mM of HU for 6 days) through in vitro assays. Viability and morphological changes were evaluated. The recovery of cysticerci\'s mobility and morphology was evaluated at 3 and 6 days, after 6 days of treatment. The number of proliferative cells was evaluated using EdU. Our results show morphological changes in the size, shape, and number of evaginated cysticerci at the 40 mM dose. The mobility of cysticerci was lower after 6 days of HU treatment at both concentrations. On days 3 and 6 of recovery after 25 mM of HU treatment, a partial recovery of the proliferative cells was observed. Proteomic and Gene Ontology analyses identified modifications in protein groups related to DNA binding, DNA damage, glycolytic enzymes, cytoskeleton, skeletal muscle, and RNA binding.
摘要：
扁虫以其非凡的再生能力而闻名，一种依赖于全能细胞的细胞，称为c虫中的发芽细胞。羟基脲（HU）耗尽发芽细胞会影响寄生虫的再生。这里,我们通过体外试验研究了HU处理（25mM和40mMHU持续6天）后，囊尾股癣中发芽细胞的减少和恢复。评价活力和形态变化。在第3天和第6天评估囊尾蚴的活动性和形态恢复，治疗6天后。使用EdU评估增殖细胞的数量。我们的结果显示了大小的形态变化，形状,和40mM剂量下消失的囊尾蚴的数量。两种浓度的HU处理6天后，囊尾蚴的迁移率较低。在25mMHU处理后恢复的第3天和第6天,观察到增殖细胞的部分恢复。蛋白质组学和基因本体论分析确定了与DNA结合相关的蛋白质组中的修饰，DNA损伤,糖酵解酶,细胞骨架,骨骼肌,和RNA结合。
