■探讨三种新鲜地黄的作用及机理,即北京3(BJ3H),淮中号.1(HZ1H),和太生(TS)通过雌激素受体途径对脂多糖(LPS)诱导的脓毒症(S-AKI)小鼠急性肾损伤模型的影响。
■BALB/c小鼠随机分为对照组(CON),模型(LPS),黄芪注射液(ASI),BJ3H,HZ1H,TS水提取物组,每组增加雌激素受体拮抗剂ICI182,780组。拮抗剂组在给药前0.5小时接受腹膜内注射ICI,并在给药后3天接受腹膜内注射LPS。肾脏病理学,函数,炎症因子,免疫细胞,活性氧(ROS)的水平,凋亡,检测小鼠肾脏TLR4/NF-κB/NLRP3信号通路蛋白表达水平。
■ASI,BJ3H,HZ1H,TS改善LPS诱导的S-AKI小鼠肾脏病理,降低肾脏和血清炎症因子水平,巨噬细胞和中性粒细胞的阳性率,ROS和细胞凋亡的水平,和TLR4,MyD88,NF-κBp-p65/NF-κBp65和NLRP3蛋白在肾脏中的相对表达水平。此外,它们增加了小鼠肾脏中树突状细胞(DC)的阳性率。HZ1H的整体效果优于ASI,BJ3H,和TS。然而,加入ICI后,药物的调节作用受到抑制。
■三种类型的新鲜谷氨酸可能通过雌激素受体途径完全或部分影响TLR4/NF-κB/NLRP3信号通路,对S-AKI发挥保护作用。
UNASSIGNED: To explore the effects and mechanism of three types of fresh Rehmannia glutinosa, namely Beijing No. 3 (BJ3H), Huaizhong No. 1 (HZ1H), and Taisheng (TS) on lipopolysaccharide (LPS)-induced acute kidney injury in the sepsis (S-AKI) mice model through the estrogen receptor pathway.
UNASSIGNED: BALB/c mice were randomly divided into control (CON), model (LPS), astragalus injection (ASI), BJ3H, HZ1H, TS water extract groups, the estrogen receptor antagonist ICI182,780 groups were added to each group. The antagonist groups received an intraperitoneal injection of ICI 0.5 hr before administration and an intraperitoneal injection of LPS 3 days after administration. The kidney pathology, function, inflammatory factors, immune cells, levels of reactive oxygen species (ROS), apoptosis, and the protein expression levels of TLR4/NF-κB/NLRP3 signaling pathway in the mice kidneys were detected.
UNASSIGNED: ASI, BJ3H, HZ1H, and TS improved LPS-induced renal pathology in S-AKI mice, reduced the kidney and serum levels of inflammatory factors, positive rates of macrophages and neutrophils, levels of ROS and apoptosis, and the relative expression levels of TLR4, MyD88, NF-κB p-p65/NF-κB p65, and NLRP3 proteins in the kidney. In addition, they increased the positive rate of dendritic cells (DCs) in the mice kidneys. The overall effect of HZ1H was superior to that of ASI, BJ3H, and TS. However, after adding ICI, the regulatory effects of drugs were inhibited.
UNASSIGNED: The three types of fresh R. glutinosa may completely or partially affect the TLR4/NF-κB/NLRP3 signaling pathway through the estrogen receptor pathway to exert a protective effect on S-AKI.