地黄具有重要的药用价值,萜类化合物是地黄的主要活性成分之一。在这项研究中,利用iTRAQ技术分析了谷氨酸根中蛋白质的相对丰度,和6,752可靠的蛋白质进行了定量。GO富集结果表明,大多数蛋白质参与了代谢过程或细胞过程,57.63%的蛋白质具有催化活性,65.80%的蛋白质富集在膜结合的细胞器中。在r.glutinosa的根中,有38个有意义的KEGG富集,在一些途径中发现了更多的DEP,特别是在根的伸长阶段和扩张阶段之间具有15.0%DEP的蛋白酶体途径和TCA周期。此外,发现了萜类化合物合成的五个KEGG途径。大多数异戊二烯基转移酶属于FPP/GPB合酶家族,参与萜类骨架的生物合成,并且都与生物素羧化酶CAC2相互作用。与伸长阶段相比,许多异戊烯基转移酶在根的扩增阶段或成熟期表现出更高的表达。此外,从谷草中克隆了8个FPP/GPB合酶编码基因,即FPPS,FPPS1,GGPS,GGPS3,GGPS4,GGPS5,GPPS和GPPS2,内含子也在FPPS中发现,FPPS1,GGPS5和GGPS2,以及FPP/3GPP合酶在生物体中较为保守,特别是在病毒科,其中GPPS或GPPS2在植物中的共现率显著较高。进一步分析发现,谷草的FPP/GPB合成酶分为三种,GGPS,GPPS和FPPS,它们的基因表达在不同品种中显著不同,生长期,或者是glutinosa的组织.与GGPS相比,GPPS和FPPS的表达在glutinosa中明显更高,尤其是在扩张阶段和成熟期。因此,芦苇根中萜类化合物的合成调控复杂,有待进一步研究。
Rehmannia glutinosa has important medicinal value; terpenoid is one of the main active components in R. glutinosa. In this
study, iTRAQ technique was used to analyze the relative abundance of proteins in roots of R. glutinosa, and 6,752 reliable proteins were quantified. GO enrichment results indicated that most proteins were involved in metabolic process or cellular process, 57.63% proteins had catalytic activity, and 65.80% proteins were enriched in membrane-bounded organelle. In roots of R. glutinosa, there were 38 KEGG enrichments with significance, more DEPs were found in some pathways, especially the proteasome pathway and TCA cycle with 15.0% DEPs between elongation stage and expansion stage of roots. Furthermore, five KEGG pathways of terpenoid synthesis were found. Most prenyltransferases belong to FPP/GGPP synthase family, involved in terpenoid backbone biosynthesis, and all interacted with biotin carboxylase CAC2. Compared with that at the elongation stage, many prenyltransferases exhibited higher expression at the expansion stage or maturation stage of roots. In addition, eight FPP/GGPP synthase encoding genes were cloned from R. glutinosa, namely FPPS, FPPS1, GGPS, GGPS3, GGPS4, GGPS5, GPPS and GPPS2, introns were also found in FPPS, FPPS1, GGPS5 and GGPS2, and FPP/GPP synthases were more conservative in organisms, especially in viridiplantae, in which the co-occurrence of GPPS or GPPS2 was significantly higher in plants. Further analysis found that FPP/GGPP synthases of R. glutinosa were divided into three kinds, GGPS, GPPS and FPPS, and their gene expression was significantly diverse in different varieties, growth periods, or tissues of R. glutinosa. Compared with that of GGPS, the expression of GPPS and FPPS was much higher in R. glutinosa, especially at the expansion stage and maturation stage. Thus, the synthesis of terpenoids in roots of R. glutinosa is intricately regulated and needs to be further studied.