Pueraria

葛根
  • 文章类型: Journal Article
    在印度东部,葛根的块茎(威尔德。)DC。因其广泛的药用和营养价值而被种族社区使用,特别是恢复牲畜健康和治疗蠕虫病。该研究旨在评估结核分枝杆菌作为驱虫药的民族兽医学重要性,验证其无毒性质,并通过计算机分子对接技术鉴定最有效的植物成分。使用八个定量指标对从185个线人收集的民族医学数据进行了定量分析,以突出使用多样性和植物中最常用的部分。采用的某些指数得分很高,如使用值(UV=0.52),保真度水平(FL=68.42%)和组织重要性值(TIV=1)清楚地说明了有关块茎部分用于治疗兽用动物肠道蠕虫病的医学营养益处的种族医学线索。基于这个民族指导的线索,块根已经通过对总酚的估计进行了化学分析研究,黄酮类化合物,单宁和生物碱,以及HPLC和GC-MS分析。通过对畜禽鸟类的某些蠕虫的体外研究,用块茎提取物评估了驱虫性能,它显示了对测试的寄生虫,即棉草,四角拉利蒂娜和塞洛里亚。使用Vero细胞和BALB/c小鼠通过体外和体内方法进行块茎提取物的毒性评估。即使在较高的实验剂量下,也观察到所研究的块茎提取物的无毒性质。在通过GC-MS分析鉴定的12种植物化合物中,一种化合物[Morphinan-4,5-环氧-3,6-二醇,6-(7-硝基苯并呋喃-4-基)氨基-]在最低结合能值的成本中表现出最佳的结合构象,其中包括一种抗炎药,一种抗氧化剂,和四种驱虫蛋白.我们的研究结果非常令人鼓舞,可以进一步深入评估这种块茎药物,以开发驱虫兽药。
    In eastern India, the tubers of Pueraria tuberosa (Willd.) DC. are used by the ethnic communities for its wide range of medicinal and nutritional value, especially to rejuvenate livestock health and to treat helminthiasis. The study is aimed to evaluate the ethnoveterinary medicinal importance of P. tuberosa as anthelmintic, to verify its nontoxic nature and identify the most potent phytoconstituents aided by in silico molecular docking technique. Ethnomedicinal data collected from 185 informants were quantitatively analyzed employing eight quantitative indices to highlight the use diversity and most frequently used part of the plant. High scores of certain indices employed, such as Use Value (UV = 0.52), Fidelity Level (FL = 68.42%) and Tissue Importance Value (TIV = 1) clearly illustrate an ethnomedicinal lead regarding medico-nutritional benefits of the tuber part used against intestinal helminthic diseases of veterinary animals. Based on this ethno-guided lead, root tuber has been investigated for its chemical profiling by the estimation of total phenolics, flavonoids, tannins and alkaloids, along with HPLC and GC-MS analyses. Anthelmintic property was evaluated with the tuber extracts by in vitro studies on some helminths of livestock and poultry birds, and it showed promising results against the tested parasites namely Cotylophoron cotylophorum, Raillietina tetragona and Setaria cervi. Toxicity assessments of tuber extract through in vitro and in vivo methods were performed using Vero cells and BALB/c mice. Nontoxic nature of the studied tuber extract was observed even in higher experimental doses. Out of 12 phytocompounds identified by GC-MS analysis, one compound [Morphinan-4,5-epoxy-3,6-di-ol,6- (7-nitrobenzofurazan-4-yl) amino-] exhibited the best binding conformations in cost of the lowest binding energy values with six target proteins that include one anti-inflammatory, one antioxidant, and four anthelmintic proteins. The findings of our study are found very encouraging to evaluate this tuber drug furthermore intensively towards the development of anthelmintic veterinary medicine.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    晶体类型是淀粉的重要物理化学性质。然而,目前尚不清楚晶体类型的变化是否会影响淀粉的其他性质。这项研究发现,水分亏缺导致葛根的小淀粉颗粒和透明度增加。thomsonii,同时导致直链淀粉含量和溶胀力降低。此外,失水条件下P.Thomsonii淀粉的晶型由CB型转变为CA型,而不会显著改变淀粉的短程有序结构和链长分布。晶体类型的这种转变导致淀粉的DSC热流曲线中的峰分裂,糊化行为的改变,和抗性淀粉含量的增加。淀粉颗粒的晶体结构和理化性质的这些变化被认为是P.Thomsonii用于应对水分亏缺的适应性策略。
    Crystal type is an important physicochemical property of starch. However, it is currently unclear whether changes in crystal type affect other properties of starch. This study discovered that water deficit resulted in an increase in small starch granules and transparency in Pueraria lobata var. thomsonii, while causing a decrease in amylose content and swelling power. Additionally, the crystal type of P. Thomsonii starch changed from CB-type to CA-type under water deficit, without significantly altering the short-range ordered structure and chain length distribution of starch. This transformation in crystal type led to peak splitting in the DSC heat flow curve of starch, alterations in gelatinization behavior, and an increase in resistant starch content. These changes in crystalline structure and physicochemical properties of starch granules are considered as adaptive strategies employed by P. Thomsonii to cope with water deficit.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    葛根(P.lobata),一种传统的抗糖尿病药物,主要由黄酮类和异黄酮组成,糖尿病治疗在中国有着悠久的历史。然而,抗糖尿病活性成分尚不清楚.最近,蛋白酪氨酸磷酸酶1B(PTP1B)通过负向调节胰岛素信号通路成为热门的治疗靶点。在这项研究中,首先采用谱效关系分析方法鉴定了抑制PTP1B的P.bobata活性成分。采用高效液相色谱法(HPLC)建立了12批样品的指纹图谱,并确定了60个常见峰。同时,通过与标准品的比较,确定了12种成分.通过使用对硝基苯酚方法在体外研究了PTP1B活性的抑制作用,和偏最小二乘判别分析,灰色关联分析,双变量相关分析,并采用聚类分析对P.lobata中的生物活性化合物进行分析。峰6,9(缩水甘油),11(生苷),12(4'-甲氧基葛根素),25、34、35、36、53和59被认为是抑制PTP1B的潜在活性物质。体外PTP1B抑制活性由缩水甘油证实,Genistin,和4'-甲氧基葛根素。三种化合物的IC50为10.56±0.42μg/mL,16.46±0.29μg/mL,和9.336±0.56μg/mL,分别,表明明显的PTP1B抑制活性。简而言之,我们建立了一种有效的方法来鉴定假单胞菌中的PTP1B酶抑制剂,这有助于阐明假单胞菌对糖尿病的物质基础。此外,显然,光谱-效应关系方法是识别活性化合物的有效方法,本研究也可为中药中生物活性成分的筛选提供参考。
    Pueraria lobata (P. lobata), a traditional anti-diabetic medicine mainly composed of flavonoids and isoflavones, has a long history in diabetes treatment in China. However, the anti-diabetic active component is still unclear. Recently, protein tyrosine phosphatase 1B (PTP1B) has been a hot therapeutic target by negatively regulating insulin signaling pathways. In this study, the spectrum-effect relationship analysis method was first used to identify the active components of P. lobata that inhibit PTP1B. The fingerprints of 12 batches of samples were established using high-performance liquid chromatography (HPLC), and sixty common peaks were identified. Meanwhile, twelve components were identified by a comparison with the standards. The inhibition of PTP1B activity was studied in vitro by using the p-nitrophenol method, and the partial least squares discriminant analysis, grey relational analysis, bivariate correlation analysis, and cluster analysis were used to analyze the bioactive compounds in P. lobata. Peaks 6, 9 (glycitin), 11 (genistin), 12 (4\'-methoxypuerarin), 25, 34, 35, 36, 53, and 59 were considered as potentially active substances that inhibit PTP1B. The in vitro PTP1B inhibitory activity was confirmed by glycitin, genistin, and 4\'-methoxypuerarin. The IC50s of the three compounds were 10.56 ± 0.42 μg/mL, 16.46 ± 0.29 μg/mL, and 9.336 ± 0.56 μg/mL, respectively, indicating the obvious PTP1B inhibitory activity. In brief, we established an effective method to identify PTP1B enzyme inhibitors in P. lobata, which is helpful in clarifying the material basis of P. lobata on diabetes. Additionally, it is evident that the spectrum-effect relationship method serves as an efficient approach for identifying active compounds, and this study can also serve as a reference for screening bioactive constituents in traditional Chinese medicine.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    蒙大拿州葛是具有重要药用价值和复杂遗传背景的物种。在这项研究中,我们对蒙大拿州两个品种的线粒体(mt)基因组进行了测序和组装。蒙大拿州变种的mt基因组长度。thomsonii和P.montanavar.蒙大拿州分别为457,390bp和456,731bp,分别。两个蒙大拿有丝分裂基因组都显示出由两个环状分子组成的多分支结构,有56个基因注释,由33个蛋白质编码基因组成,18个tRNA基因(trnC-GCA和trnM-CAU是多拷贝基因),和3个rRNA基因。然后,在蒙大拿州假单胞菌的mt基因组中检测到207对长重复序列和96个简单序列重复序列(SSR),在每个品种的33个线粒体蛋白质编码基因中发现了484个潜在的RNA编辑位点。此外,同伦序列分析显示两个mt基因组之间高度共线性。这项工作是第一个分析蒙大拿州的mt基因组。它可以提供可用于分析高等植物mt基因组结构的信息,并为未来相关物种的比较基因组研究和进化生物学研究提供基础。
    Pueraria montana is a species with important medicinal value and a complex genetic background. In this study, we sequenced and assembled the mitochondrial (mt) genomes of two varieties of P. montana. The mt genome lengths of P. montana var. thomsonii and P. montana var. montana were 457,390 bp and 456,731 bp, respectively. Both P. montana mitogenomes showed a multi-branched structure consisting of two circular molecules, with 56 genes annotated, comprising 33 protein-coding genes, 18 tRNA genes (trnC-GCA and trnM-CAU are multi-copy genes), and 3 rRNA genes. Then, 207 pairs of long repeats and 96 simple sequence repeats (SSRs) were detected in the mt genomes of P. montana, and 484 potential RNA-editing sites were found across the 33 mitochondrial protein-coding genes of each variety. Additionally, a syntenic sequence analysis showed a high collinearity between the two mt genomes. This work is the first to analyze the mt genomes of P. montana. It can provide information that can be used to analyze the structure of mt genomes of higher plants and provide a foundation for future comparative genomic studies and evolutionary biology research in related species.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    葛根(P.lobata),一种双重用途的食品和药品,在酒精解毒和肝脏保护方面表现出有限的功效,与以前的研究主要集中在葛根素干。在这项研究中,我们研究了新鲜的根源性外泌体样纳米囊泡(P-ELNs)减轻酒精中毒的潜在作用和机制,对C57BL/6J小鼠具有促进酒精代谢和保护肝脏的作用。
    我们使用差速离心从新鲜的P.lobata根中分离了P-ELN,并通过透射电子显微镜对其进行了表征,纳米级粒度,ζ电位分析,和生化化验。在用P-ELN预处理的急性酒精中毒(AAI)小鼠中,我们评估了它们对正确反射(LORR)丧失的时间和持续时间的影响,肝脏酒精代谢酶活性,肝脏和血清酒精含量,和铁凋亡相关标记。
    P-ELN,富含蛋白质,脂质,和小RNA,表现出理想的尺寸(150.7±82.8nm)和负表面电荷(-31mV)。用10mg/(kg。bw)雄性和雌性小鼠的P-ELN显着延长了焦虑时间,缩短了清醒时间,增强乙醛脱氢酶(ALDH)活性,同时抑制乙醇脱氢酶(ADH)活性,肝脏和血清中的酒精含量降低。值得注意的是,与P-ELNs上清液(丰富的葛根素含量)相比,P-ELNs表现出更高的功效,提示葛根素以外的其他活性成分。此外,P-ELN通过抑制谷胱甘肽过氧化物酶4(GPX4)和还原型谷胱甘肽(GSH)的还原来防止铁死亡,并抑制酰基辅酶A合成酶长链家族成员4(ACSL4)的升高,从而减轻病理性肝脏脂质积累。
    P-ELNs表现出独特的外泌体特征并有效缓解酒精中毒,改善酒精代谢,抑制铁性凋亡,保护肝脏免受酒精损伤。因此,P-ELN有望成为解毒的治疗剂,清醒促进,预防酒精性肝损伤。
    UNASSIGNED: Pueraria lobata (P. lobata), a dual-purpose food and medicine, displays limited efficacy in alcohol detoxification and liver protection, with previous research primarily focused on puerarin in its dried roots. In this study, we investigated the potential effects and mechanisms of fresh P. lobata root-derived exosome-like nanovesicles (P-ELNs) for mitigating alcoholic intoxication, promoting alcohol metabolism effects and protecting the liver in C57BL/6J mice.
    UNASSIGNED: We isolated P-ELNs from fresh P. lobata root using differential centrifugation and characterized them via transmission electron microscopy, nanoscale particle sizing, ζ potential analysis, and biochemical assays. In Acute Alcoholism (AAI) mice pre-treated with P-ELNs, we evaluated their effects on the timing and duration of the loss of the righting reflex (LORR), liver alcohol metabolism enzymes activity, liver and serum alcohol content, and ferroptosis-related markers.
    UNASSIGNED: P-ELNs, enriched in proteins, lipids, and small RNAs, exhibited an ideal size (150.7 ± 82.8 nm) and negative surface charge (-31 mV). Pre-treatment with 10 mg/(kg.bw) P-ELNs in both male and female mice significantly prolonged ebriety time, shortened sobriety time, enhanced acetaldehyde dehydrogenase (ALDH) activity while concurrently inhibited alcohol dehydrogenase (ADH) activity, and reduced alcohol content in the liver and serum. Notably, P-ELNs demonstrated more efficacy compared to P-ELNs supernatant fluid (abundant puerarin content), suggesting alternative active components beyond puerarin. Additionally, P-ELNs prevented ferroptosis by inhibiting the reduction of glutathione peroxidase 4 (GPX4) and reduced glutathione (GSH), and suppressing acyl-CoA synthetase long-chain family member 4 (ACSL4) elevation, thereby mitigating pathological liver lipid accumulation.
    UNASSIGNED: P-ELNs exhibit distinct exosomal characteristics and effectively alleviate alcoholic intoxication, improve alcohol metabolism, suppress ferroptosis, and protect the liver from alcoholic injury. Consequently, P-ELNs hold promise as a therapeutic agent for detoxification, sobriety promotion, and prevention of alcoholic liver injury.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    蒙大拿州葛根var。lobata(P.lobata)是一种传统的药用植物,属于豆科的葛属。蒙大拿州葛根var。thomsonii(P.thomsonii)和蒙大拿州葛根。蒙大拿州(P.蒙大拿州)是其相关物种。然而,葛属的进化史在很大程度上仍然未知。这里,高度诚信,报道了P.lob的染色体水平基因组和改进的P.thomsonii基因组。它发现了三个葛属物种中古老的全基因组重复和最近的全基因组重复的证据。121份葛根种质的种群基因组学研究表明,葛根种群具有较高的遗传多样性,和P。thomsonii可能是通过驯化为亚种而衍生自P。选择扫描分析确定了与生长素和赤霉素合成相关的Thomsonii种群中的候选基因,这可能在P.thomsonii的块茎的膨胀和淀粉积累中起作用。总的来说,这些发现为葛根基因组的进化和驯化历史提供了新的见解,并为这些物种的遗传改良提供了宝贵的基因组资源。
    Pueraria montana var. lobata (P. lobata) is a traditional medicinal plant belonging to the Pueraria genus of Fabaceae family. Pueraria montana var. thomsonii (P. thomsonii) and Pueraria montana var. montana (P. montana) are its related species. However, evolutionary history of the Pueraria genus is still largely unknown. Here, a high-integrity, chromosome-level genome of P. lobata and an improved genome of P. thomsonii were reported. It found evidence for an ancient whole-genome triplication and a recent whole-genome duplication shared with Fabaceae in three Pueraria species. Population genomics of 121 Pueraria accessions demonstrated that P. lobata populations had substantially higher genetic diversity, and P. thomsonii was probably derived from P. lobata by domestication as a subspecies. Selection sweep analysis identified candidate genes in P. thomsonii populations associated with the synthesis of auxin and gibberellin, which potentially play a role in the expansion and starch accumulation of tubers in P. thomsonii. Overall, the findings provide new insights into the evolutionary and domestication history of the Pueraria genome and offer a valuable genomic resource for the genetic improvement of these species.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    黄酮类化合物,多种植物次生代谢产物,以其多样化的生物活性而闻名。异黄酮是类黄酮的一个亚类,因其潜在的健康益处而受到关注。葛根素是在葛根和葛属中发现的生物活性异黄酮之一,它被广泛用于替代中药,并被发现可以有效治疗心血管疾病等慢性病,肝脏疾病,胃病,呼吸系统疾病,糖尿病,老年痴呆症,和癌症。在过去的几年中,葛根素已被广泛研究并用于科学和临床研究。这篇综述的目的是提供葛根素生物合成的最新探索,最常见的提取方法,分析技术,和生物效应,有可能为医药研发提供新的视角。
    Flavonoids, a variety of plant secondary metabolites, are known for their diverse biological activities. Isoflavones are a subgroup of flavonoids that have gained attention for their potential health benefits. Puerarin is one of the bioactive isoflavones found in the Kudzu root and Pueraria genus, which is widely used in alternative Chinese medicine, and has been found to be effective in treating chronic conditions like cardiovascular diseases, liver diseases, gastric diseases, respiratory diseases, diabetes, Alzheimer\'s disease, and cancer. Puerarin has been extensively researched and used in both scientific and clinical studies over the past few years. The purpose of this review is to provide an up-to-date exploration of puerarin biosynthesis, the most common extraction methods, analytical techniques, and biological effects, which have the potential to provide a new perspective for medical and pharmaceutical research and development.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    肠道菌群功能紊乱是影响慢性肾脏病(CKD)易感性的关键因素。葛根(PLR),一种中药和食物同源的草药,已知促进肠道微生物群稳态;然而,它在肾脏保护中的作用仍然未知。本研究旨在探讨PLR缓解CKD的疗效和潜在机制。应用8周2%NaCl喂养小鼠模型诱导CKD并评估PLR补充的治疗效果。灌胃8周后,中、高剂量PLR可显着缓解CKD相关肌酐,尿蛋白增多和肾病组织病理学损伤。此外,PLR通过减少炎症反应和下调经典Wnt/β‑catenin通路保护肾脏免受纤维化。此外,PLR拯救了肠道微生物群生态失调,并防止了高盐诱导的肠道屏障功能障碍。PLR干预后发现Akkermansia和双歧杆菌的富集,其相对丰度与肾脏组织学和功能的正常维持呈正相关。接下来,粪便菌群移植实验验证了PLR对CKD的积极作用,至少部分地,通过肠道微生物群的重建和Wnt/β‑catenin途径的下调而发挥作用。本研究为PLR在肾脏保护中的新功能提供了证据,并提出了潜在的CKD治疗策略靶点。
    Gut microbiota dysfunction is a key factor affecting chronic kidney disease (CKD) susceptibility. Puerariae lobatae Radix (PLR), a traditional Chinese medicine and food homologous herb, is known to promote the gut microbiota homeostasis; however, its role in renoprotection remains unknown. The present study aimed to investigate the efficacy and potential mechanism of PLR to alleviate CKD. An 8‑week 2% NaCl‑feeding murine model was applied to induce CKD and evaluate the therapeutic effect of PLR supplementary. After gavage for 8 weeks, The medium and high doses of PLR significantly alleviated CKD‑associated creatinine, urine protein increasement and nephritic histopathological injury. Moreover, PLR protected kidney from fibrosis by reducing inflammatory response and downregulating the canonical Wnt/β‑catenin pathway. Furthermore, PLR rescued the gut microbiota dysbiosis and protected against high salt‑induced gut barrier dysfunction. Enrichment of Akkermansia and Bifidobacterium was found after PLR intervention, the relative abundances of which were in positive correlation with normal maintenance of renal histology and function. Next, fecal microbiota transplantation experiment verified that the positive effect of PLR on CKD was, at least partially, exerted through gut microbiota reestablishment and downregulation of the Wnt/β‑catenin pathway. The present study provided evidence for a new function of PLR on kidney protection and put forward a potential therapeutic strategy target for CKD.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    代谢综合征(MetS)是心血管疾病(CVD)的一组危险因素,已成为全球公共卫生问题。葛根素(PUE),葛根的主要活性化合物,具有调节糖脂代谢和防止心血管损害的作用。这项研究旨在调查膳食补充PUE是否可以改善MetS及其相关的心血管损害。大鼠随机分为三组:正常饮食组(NC),高脂肪/高糖饮食组(HFHS),和HFHS加PUE饮食组(HFHS-PUE)。结果表明,补充PUE的大鼠表现出增强的糖耐量,改善脂质参数,与单独使用HFHS饮食的人相比,血压降低。此外,PUE逆转了HFHS诱导的动脉粥样硬化指数(AI)以及血清乳酸脱氢酶(LDH)和肌酸激酶(CK)活性的升高。超声评估表明PUE显着改善了心功能不全和动脉僵硬度。组织病理学评估进一步证实,PUE可显着减轻心脏重塑,动脉重塑,和大脑中的神经元损伤。此外,PUE降低全身炎症指标,包括C反应蛋白(CRP),中性粒细胞与淋巴细胞比率(NLR),单核细胞与淋巴细胞比率(MLR),和全身免疫炎症指数(SII)。总之,膳食补充PUE可有效缓解代谢紊乱,减轻全身性炎症,最大限度地减少了HFHS饮食诱导的MetS大鼠的心血管损伤。这些结果为膳食PUE补充对预防和管理MetS及其相关CVD的潜在益处提供了新的见解。
    Metabolic syndrome (MetS) is a cluster of risk factors for cardiovascular diseases (CVDs) that has become a global public health problem. Puerarin (PUE), the principal active compound of Pueraria lobata, has the effects of regulating glucose and lipid metabolism and protecting against cardiovascular damage. This study aimed to investigate whether dietary supplementation with PUE could ameliorate MetS and its associated cardiovascular damage. Rats were randomly divided into three groups: the normal diet group (NC), the high-fat/high-sucrose diet group (HFHS), and the HFHS plus PUE diet group (HFHS-PUE). The results showed that PUE-supplemented rats exhibited enhanced glucose tolerance, improved lipid parameters, and reduced blood pressure compared to those on the HFHS diet alone. Additionally, PUE reversed the HFHS-induced elevations in the atherogenic index (AI) and the activities of serum lactate dehydrogenase (LDH) and creatine kinase (CK). Ultrasonic evaluations indicated that PUE significantly ameliorated cardiac dysfunction and arterial stiffness. Histopathological assessments further confirmed that PUE significantly mitigated cardiac remodeling, arterial remodeling, and neuronal damage in the brain. Moreover, PUE lowered systemic inflammatory indices including C-reactive protein (CRP), neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), and systemic immune-inflammation index (SII). In conclusion, dietary supplementation with PUE effectively moderated metabolic disorders, attenuated systemic inflammation, and minimized cardiovascular damage in rats with MetS induced by an HFHS diet. These results provide novel insights into the potential benefits of dietary PUE supplementation for the prevention and management of MetS and its related CVDs.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

  • 文章类型: Journal Article
    形成异黄酮骨架结构的途径主要限于豆科。通过定制酶在复合主链上的后续装饰将改变其生物学和药用特性。葛属是一种豆科植物,作为传统中药,其根源被赋予了许多药理活性。糖基化和甲基化是根中异黄酮代谢的主要修饰过程。导致独特的糖基化和甲基化末端异黄酮化合物的积累。例如,大豆苷元8-C-葡萄糖苷(即,葛根素)和葛根素衍生物仅由葛属产生。葛根素已被确立为治疗心血管疾病的临床药物。为了更好地了解P中异黄酮的特征性代谢,本文综述了近年来国内外关于假单胞菌异黄酮主要糖基化和甲基化及其生物合成酶的研究进展。
    The pathway for forming isoflavonoid skeletal structure is primarily restricted to the Leguminosae family. Subsequent decorations on the compound backbone by tailoring enzymes would change their biological and medicinal properties. Pueraria lobata is a leguminous plant, and as a traditional Chinese medicine its roots have been ascribed a number of pharmacological activities. Glycosylation and methylation are the main modifying processes in isoflavonoid metabolism in P. lobata roots, resulting in the accumulation of unique glycosylated and methylated end isoflavonoid compounds. For instance, daidzein 8-C-glucoside (i.e., puerarin) and puerarin derivatives are produced only by the Pueraria genus. Puerarin has been established as a clinical drug for curing cardiovascular diseases. To better understand the characteristic isoflavonoid metabolism in P. lobata, this review attempts to summarize the research progress made with understanding the main glycosylation and methylation of isoflavonoids in P. lobata and their biosynthetic enzymes.
    导出

    更多引用

    收藏

    翻译标题摘要

    我要上传

       PDF(Pubmed)

公众号