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Abstract：
Pueraria montana is a species with important medicinal value and a complex genetic background. In this study, we sequenced and assembled the mitochondrial (mt) genomes of two varieties of P. montana. The mt genome lengths of P. montana var. thomsonii and P. montana var. montana were 457,390 bp and 456,731 bp, respectively. Both P. montana mitogenomes showed a multi-branched structure consisting of two circular molecules, with 56 genes annotated, comprising 33 protein-coding genes, 18 tRNA genes (trnC-GCA and trnM-CAU are multi-copy genes), and 3 rRNA genes. Then, 207 pairs of long repeats and 96 simple sequence repeats (SSRs) were detected in the mt genomes of P. montana, and 484 potential RNA-editing sites were found across the 33 mitochondrial protein-coding genes of each variety. Additionally, a syntenic sequence analysis showed a high collinearity between the two mt genomes. This work is the first to analyze the mt genomes of P. montana. It can provide information that can be used to analyze the structure of mt genomes of higher plants and provide a foundation for future comparative genomic studies and evolutionary biology research in related species.
摘要：
蒙大拿州葛是具有重要药用价值和复杂遗传背景的物种。在这项研究中,我们对蒙大拿州两个品种的线粒体（mt）基因组进行了测序和组装。蒙大拿州变种的mt基因组长度。thomsonii和P.montanavar.蒙大拿州分别为457,390bp和456,731bp，分别。两个蒙大拿有丝分裂基因组都显示出由两个环状分子组成的多分支结构，有56个基因注释，由33个蛋白质编码基因组成，18个tRNA基因（trnC-GCA和trnM-CAU是多拷贝基因），和3个rRNA基因。然后，在蒙大拿州假单胞菌的mt基因组中检测到207对长重复序列和96个简单序列重复序列（SSR），在每个品种的33个线粒体蛋白质编码基因中发现了484个潜在的RNA编辑位点。此外,同伦序列分析显示两个mt基因组之间高度共线性。这项工作是第一个分析蒙大拿州的mt基因组。它可以提供可用于分析高等植物mt基因组结构的信息，并为未来相关物种的比较基因组研究和进化生物学研究提供基础。
