背景:巴贝虫。是感染驯养动物红细胞的原生动物寄生虫,野生动物和人类。几例大熊猫(就野生动物保护而言是旗舰物种)感染了假定的新型巴贝斯虫。已被报道。然而,对这种新型Babesiasp的形态和分子分类学分类进行了全面研究。仍然缺乏。这项研究旨在弥补这一差距,并正式描述这种新的Babesiasp。感染大熊猫.
方法:详细的形态学,进行了分子和系统发育分析,以表征这种Babesiasp。并评估其与其他Babesiaspp的系统关系。对感染巴贝虫的大熊猫的血液样本进行显微镜检查。18S核糖体RNA(18SrRNA),新巴贝斯虫的细胞色素b(cytb)和线粒体基因组(有丝分裂基因组)。被放大,使用从受感染大熊猫的血液样本中纯化的DNA进行测序和组装。基于新产生的18SrRNA,cytb和有丝分裂基因组序列,构建了系统发育树。
结果:形态学,巴贝西亚sp.从大熊猫展示的各种形态,包括圆形到椭圆形的环形形态,类似于在其他小犬巴贝斯虫属物种中发现的那些。并显示典型的四分体。用18SrRNA进行系统发育分析,cytb和有丝分裂基因组序列揭示了新的Babesiasp。形成一个单系群,与巴贝虫属物种有密切的系统发育关系。感染熊(Ursidae),浣熊(原科)和犬科(犬科)。值得注意的是,有丝分裂基因组结构由六个核糖体大亚基编码基因(LSU1-6)和三个蛋白质编码基因(cytb,cox3和cox1)线性排列。
结论:基于耦合的形态和遗传分析,我们描述了一种新的Babesia属物种,即,ailuropodaen.sp.,感染大熊猫。
BACKGROUND: Babesia spp. are protozoan parasites that infect the red blood cells of domesticated animals, wildlife and humans. A few cases of giant pandas (a flagship species in terms of wildlife conservation) infected with a putative novel Babesia sp. have been reported. However, comprehensive research on the morphological and molecular taxonomic classification of this novel Babesia sp. is still lacking. This study was designed to close this gap and formally describe this new Babesia sp. infecting giant pandas.
METHODS: Detailed morphological, molecular and phylogenetic analyses were conducted to characterise this Babesia sp. and to assess its systematic relationships with other Babesia spp. Blood samples from giant pandas infected with Babesia were subjected to microscopic examination. The 18S ribosomal RNA (18S rRNA), cytochrome b (cytb) and mitochondrial genome (mitogenome) of the new Babesia sp. were amplified, sequenced and assembled using DNA purified from blood samples taken from infected giant pandas. Based on the newly generated 18S rRNA, cytb and mitogenome sequences, phylogenetic trees were constructed.
RESULTS: Morphologically, the Babesia sp. from giant pandas exhibited various forms, including round to oval ring-shaped morphologies, resembling those found in other small canine Babesia spp. and displaying typical tetrads. Phylogenetic analyses with the 18S rRNA, cytb and mitogenome sequences revealed that the new Babesia sp. forms a monophyletic group, with a close phylogenetic relationship with the Babesia spp. that infect bears (Ursidae), raccoons (Procyonidae) and canids (Canidae). Notably, the mitogenome structure consisted of six ribosomal large subunit-coding genes (LSU1-6) and three protein-coding genes (cytb, cox3 and cox1) arranged linearly.
CONCLUSIONS: Based on coupled morphological and genetic analyses, we describe a novel species of the genus Babesia, namely, Babesia ailuropodae n. sp., which infects giant pandas.