PDF(Pubmed)
Abstract：
A Gram-negative, non-motile, and creamy-white coloured bacterium, designated CAU 1616T, was isolated from sea sand collected at Ayajin Beach, Goseong-gun, Republic of Korea. The bacterium was found to grow optimally at 37 °C, pH 8.0-8.5, and with 1-5 % (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain CAU 1616T within the order Rhodospirillales. The highest 16S rRNA gene sequence similarity was to Fodinicurvata fenggangensis YIM D812T (94.1 %), Fodinicurvata sediminis YIM D82T (93.7 %), Fodinicurvata halophila BA45ALT (93.6 %) and Algihabitans albus HHTR 118T (92.3 %). Comparing strain CAU 1616T with closely related species (Fodinicurvata fenggangensis YIM D812T and Fodinicurvata sediminis YIM D82T), the average nucleotide identity based on blast+ values were 69.7-69.8 %, the average amino acid identity values were 61.3-61.4 %, and the digital DNA-DNA hybridization values were 18.4-18.5 %. The assembled draft genome of strain CAU 1616T had 29 contigs with an N50 value of 385.8 kbp, a total length of 3 490 371 bp, and a DNA G+C content of 65.1 mol%. The predominant cellular fatty acids were C18 : 1 2-OH, C19 : 0 cyclo ω8c, and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c). The major respiratory quinone was Q-10. Based on phenotypic, phylogenetic, and chemotaxonomic evidence, strain CAU 1616T represents a novel genus in the family Rhodovibrionaceae, for which the name Aquibaculum arenosum gen. nov., sp. nov. is proposed. The type strain is CAU 1616T (=KCTC 82428T=MCCC 1K06089T).
摘要：
革兰氏阴性，不活动，和乳白色细菌，指定CAU1616T，是从阿亚津滩收集的海沙中分离出来的，Goseong-gun,大韩民国。发现细菌在37°C时生长最佳，pH8.0-8.5,和1-5%(w/v)NaCl。基于16SrRNA基因序列的系统发育分析将菌株CAU1616T置于红螺螺旋藻目范围内。16SrRNA基因序列相似性最高的是FodinicurvataFenggangensisYIMD812T（94.1％），D82T(93.7%)，嗜盐BA45ALT(93.6%)和海藻HHTR118T(92.3%)。将菌株CAU1616T与密切相关的物种（FodinicurvataFenggangenensisYIMD812T和FodinicurvataseemminisYIMD82T）进行比较，基于blast+值的平均核苷酸同一性为69.7-69.8%，平均氨基酸同一性值为61.3-61.4%，数字DNA-DNA杂交值为18.4-18.5%。CAU1616T菌株的组装基因组草案具有29个重叠群，N50值为385.8kbp，总长度为3490371bp，DNAG+C含量为65.1mol%。主要的细胞脂肪酸是C18：12-OH，C19：0循环ω8c，和求和特征8(C18:1ω6c和/或C18:1ω7c)。主要的呼吸醌是Q-10。基于表型，系统发育，和化学分类学证据，菌株CAU1616T代表了红弧菌科中的一个新属，命名为Aquibaculumarenosumgen。11月。,sp.11月。是提议的。菌株类型为CAU1616T(=KCTC82428T=MCCC1K06089T)。
