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Abstract：
Demequina, commonly found in coastal and marine environments, represents a genus of Actinomycetes. In this study, strains Demequina PMTSA13T and OYTSA14 were isolated from the rhizosphere of Capsicum annuum, leading to the discovery of a novel species, Demequina capsici. Bacteria play a significant role in plant growth, yet there have been no reports of the genus Demequina acting as plant growth-promoting bacteria (PGPB). Comparative genomics analysis revealed ANI similarity values of 74.05-80.63% for PMTSA13T and 74.02-80.54% for OYTSA14, in comparison to various Demequina species. The digital DNA-DNA hybridization (dDDH) values for PMTSA13T ranged from 19 to 39%, and 19.1-38.6% for OYTSA14. Genome annotation revealed the presence of genes associated with carbohydrate metabolism and transport, suggesting a potential role in nutrient cycling and availability for plants. These strains were notably rich in genes related to \'carbohydrate metabolism and transport (G)\', according to their Cluster of Orthologous Groups (COG) classification. Additionally, both strains were capable of producing auxin (IAA) and exhibited enzymatic activities for cellulose degradation and catalase. Furthermore, PMTSA13T and OYTSA14 significantly induced the growth of Arabidopsis thaliana seedlings primarily attributed to their capacity to produce IAA, which plays a crucial role in stimulating plant growth and development. These findings shed light on the potential roles of Demequina strains in plant-microbe interactions and agricultural applications. The type strain is Demequina capsici PMTSA13T (= KCTC 59028T = GDMCC 1.4451T), meanwhile OYTSA14 is identified as different strains of Demequina capsici.
摘要：
Demequina,常见于沿海和海洋环境，代表放线菌属。在这项研究中,从辣椒根际分离出菌株DemequinaPMTSA13T和OYTSA14，导致了一个新物种的发现，Demequinacapsici.细菌在植物生长中起着重要作用,然而，还没有关于Demequina属作为植物生长促进细菌（PGPB）的报道。比较基因组学分析显示，与各种Demequina物种相比，PMTSA13T的ANI相似性值为74.05-80.63％，OYTSA14的ANI相似性值为74.02-80.54％。PMTSA13T的数字DNA-DNA杂交（dDDH）值范围为19％至39％，OYTSA14为19.1-38.6%。基因组注释揭示了与碳水化合物代谢和运输相关的基因的存在，表明在植物的养分循环和可用性中的潜在作用。这些菌株富含与“碳水化合物代谢和运输（G）”相关的基因，根据它们的直系同源群(COG)分类。此外,这两个菌株都能够产生生长素（IAA），并表现出纤维素降解和过氧化氢酶的酶活性。此外，PMTSA13T和OYTSA14显着诱导拟南芥幼苗的生长，这主要归因于它们产生IAA的能力，在刺激植物生长发育中起着至关重要的作用。这些发现揭示了Demequina菌株在植物-微生物相互作用和农业应用中的潜在作用。类型菌株为辣椒PMTSA13T（=KCTC59028T=GDMCC1.4451T），同时，OYTSA14被鉴定为辣椒的不同菌株。
