赖氨酸在促进发展中起着至关重要的作用,增强免疫功能,改善中枢神经系统组织的功能。氨基酸的两种构型异构体具有显著不同的作用。目前,已经报道了赖氨酸的手性识别方法;然而,以前的检测方法具有诸如昂贵的设备和复杂的检测过程的缺点。荧光分析,另一方面,拥有高灵敏度,强选择性,操作简单。在这项研究中,我们合成了四种新的基于联萘胺(BINAM)的荧光探针,能够在构成人类蛋白质的20个氨基酸中特异性鉴定赖氨酸的L构型。对映体荧光增强比(ef或ΔIL/ΔID)达到15.29,显示出高对映选择性。此外,我们评估了探针在不同pH水平下的识别能力,反应时间,和金属离子条件,以及它的检测限(LOD)和量子产率。我们的结果表明,该探针可作为检测手性赖氨酸的高度稳定工具。
Lysine plays a crucial role in promoting development, enhancing immune function, and improving the function of central nervous system tissues. The two configurational isomers of amino acids have significantly different effects. Currently, methods for chiral recognition of
lysine have been reported; however, previous detection methods have drawbacks such as expensive equipment and complicated detection processes. Fluorescence analysis, on the other hand, boasts high sensitivity, strong selectivity, and simple operation. In this study, we synthesized four novel Binaphthyl-Amine (BINAM)-based fluorescent probes capable of specifically identifying the L-configuration of
lysine among the twenty amino acids that constitute human proteins. The enantiomeric fluorescence enhancement ratio (ef or ΔIL/ΔID) reached up to 15.29, demonstrating high enantioselectivity. In addition, we assessed the probe\'s recognition capabilities under varying pH levels, reaction times, and metal ion conditions, along with its limit of detection (LOD) and quantum yield. Our results suggest that this probe serves as a highly stable tool for the detection of chiral
lysine.