PDF(Pubmed)
Abstract：
German chamomile (Matricaria chamomilla L.) is an essential oil- containing medicinal plant used worldwide. The aim of this study was to gain knowledge of the phytochemical composition and the analgesic and soporific activity of Matricaria chamomilla L. (German chamomile) flower extract and its amino acid preparations, to predict the mechanisms of their effects by molecular docking and to develop aqueous printing gels and novel 3D-printed oral dosage forms for the flower extracts. In total, 22 polyphenolic compounds and 14 amino acids were identified and quantified in the M. chamomilla extracts. In vivo animal studies with rodents showed that the oral administration of such extracts revealed the potential for treating of sleep disorders and diseases accompanied by pain. Amino acids were found to potentiate these effects. Glycine enhanced the analgesic activity the most, while lysine and β-alanine improved the soporific activity. The molecular docking analysis revealed a high probability of γ-aminobutyric acid type A (GABAA) and N-methyl-D-aspartate (NMDA) receptor antagonism and 5-lipoxygenase (LOX-5) inhibition by the extracts. A polyethylene oxide (PEO)-based gel composition with the M. chamomilla extracts was proposed for preparing a novel 3D-printed dosage form for oral administration. These 3D-printed extract preparations can be used, for example, in dietary supplement applications.
摘要：
德国洋甘菊（MatricariachamomillaL.）是世界范围内使用的一种重要的含油药用植物。这项研究的目的是获得植物化学组成的知识以及MatricariachamomillaL.（德国洋甘菊）花提取物及其氨基酸制剂的镇痛和催眠活性，通过分子对接来预测其作用机制，并开发用于花提取物的水性打印凝胶和新型3D打印口服剂型。总的来说,在甘菊提取物中鉴定并定量了22种多酚化合物和14种氨基酸。用啮齿动物进行的体内动物研究表明，口服此类提取物揭示了治疗睡眠障碍和伴有疼痛的疾病的潜力。发现氨基酸增强了这些作用。甘氨酸增强镇痛活性最多，而赖氨酸和β-丙氨酸提高了催眠活性。分子对接分析显示提取物对γ-氨基丁酸A型（GABAA）和N-甲基-D-天冬氨酸（NMDA）受体拮抗作用和5-脂氧合酶（LOX-5）抑制的可能性很高。提出了具有洋甘菊提取物的基于聚环氧乙烷(PEO)的凝胶组合物用于制备用于口服给药的新型3D打印剂型。这些3D打印的提取物制剂可以使用，例如,在膳食补充剂应用中。
