■犬T区淋巴瘤(TZL)被认为是惰性CD45-T细胞淋巴瘤,具有低侵略性和高总生存率。通过组织病理学和免疫组织化学获得诊断,还通过与免疫表型相关的淋巴结细胞学检查。淋巴细胞增多通常被确定为约10,000个细胞/μl,可能达到30,760个细胞/μl。
■本报告描述了一个雌性金毛猎犬的案例,九岁,全身淋巴结病。在颈浅表淋巴结的细胞学检查中,一个小的单态种群,“透明细胞”和“手镜”淋巴细胞形状提示TZL。由于小的透明细胞淋巴细胞增多(152,048细胞/μl),白细胞图显示强烈的白细胞增多(160,050细胞/μl)。骨髓图显示髓系与红系的比例为2:3;细胞类型呈锥体分布,差异计数中存在22.8%的淋巴细胞。骨髓,外周血,淋巴结免疫分型导致淋巴细胞门有97.3%至99.5%的CD5+,主要是CD4-,CD8-,和CD45-证实TZL与相关白血病的诊断。开始用苯丁酸氮芥和泼尼松龙治疗。在第一个月,淋巴细胞数量保持在200,000个细胞/uL以上。经过四个月的治疗,淋巴细胞减少了,在第11个月逐渐达到10,800个细胞/ul的计数。
■在文献中,在TZL中未观察到超过30,760个细胞/μl的淋巴细胞增多。因此,据信这是对化疗反应缓慢的极端淋巴细胞增多的首例报道。
UNASSIGNED: Canine T-zone lymphoma (TZL) is recognized as an indolent CD45-T cell lymphoma, with low aggressiveness and high overall survival. The diagnosis is obtained by histopathology and immunohistochemistry, but also by cytological examination of the lymph node associated with immunophenotyping.
Lymphocytosis is commonly identified as around 10,000 cells/µl and may reach 30,760 cells/µl.
UNASSIGNED: The present report describes a case of a female Golden Retriever, nine years old, with generalized lymphadenopathy. In the cytological examination of the superficial cervical lymph node, a monomorphic population of small, \"clear cells\" and \"hand mirror\" lymphocyte shape was suggestive of TZL. The leukogram showed intense leukocytosis (160,050 cells/μl) due to small clear cell
lymphocytosis (152,048 cells/μl). The myelogram showed a myeloid:erythroid ratio of 2:3; with a pyramidal distribution of cell types and the presence of 22.8% of lymphocytes in the differential count. Bone marrow, peripheral blood, and lymph node immunophenotyping resulted in lymphocyte gates with 97.3% to 99.5% CD5+, predominantly CD4-, CD8-, and CD45- confirming the diagnosis of TZL with associated leukemia. Treatment with chlorambucil and prednisolone was started. During the first month, the
lymphocytosis remained above 200,000 cells/uL. After four months of treatment, there was a decrease in lymphocytes, which progressively reached a count of 10,800 cells/ul in the eleventh month.
UNASSIGNED: In the literature,
lymphocytosis above 30,760 cells/μl has not been observed in TZLs. Thus, it is believed that this is the first report of extreme
lymphocytosis with a slow response to chemotherapy.