背景:微针是微小的针头,通常长度从几十到几百微米,用于各种医疗程序和治疗。测试的医疗设备命名为“CELLADEEP贴片”可溶解微针治疗系统(MTS),由透明质酸和胶原蛋白制成。离子电渗技术也应用于该系统。该研究旨在评估“CELLADEEP贴片”在改善皮肤方面的有效性。
方法:本研究使用离体人源皮肤组织模型,将其分为三个不同的组,即,未治疗组,阴性对照组,和测试组分别。未治疗组没有接受治疗措施,阴性对照组暴露于紫外线B(UVB)照射,测试组暴露于UVB照射并用“CELLADEEP贴片”处理。皮肤水分含量,透皮失水,通过三种临床设备评估皮肤弹性。此外,还分析了组织学染色和相关的mRNA表达水平。
结果:皮肤水分含量的结果,透皮失水,和皮肤弹性评估一致表明,“CELLADEEP贴片”的应用导致显著的皮肤改善。组织学染色图像的分析也证实了“CELLADEEP贴片”的有效性,特别是增加胶原蛋白密度。此外,胶原1a(COL1A1)和透明质酸合酶3mRNA表达上调,基质金属蛋白酶1(MMP-1)和白细胞介素1β(IL-1β)mRNA表达降低反映了其皱纹的改善,保湿和抗炎功能。
结论:\"CELLADEPP修补程序\",MTS结合离子电渗技术,展示其保湿功效,改善皮肤弹性,和抗炎功能,当应用于离体的人源皮肤组织模型的实验。该研究有助于理解“CELLADEPP贴片”,并为随后的动物实验和临床试验奠定了基础。
BACKGROUND: Microneedles are tiny needles, typically ranging from tens to hundreds of micrometers in length, used in various medical procedures and treatments. The tested medical device named \"CELLADEEP Patch\" a dissolvable microneedle therapy system (MTS), made of hyaluronic acid and collagen. And the
iontophoresis technique is also applied in the system. The study aimed to evaluate the effectiveness of the \"CELLADEEP Patch\" in skin improvement.
METHODS: Ex vivo human-derived skin tissue models were used in this study and they were divided into three different groups, namely, the Untreated Group, the Negative Control Group, and the Test Group respectively. The Untreated Group received no treatment measures, the Negative Control Group was exposed to ultraviolet B radiation (UVB) irradiation, and the Test Group was exposed to UVB irradiation and treated with \"CELLADEEP Patch\". Skin moisture content, transdermal water loss, and skin elasticity were evaluated by three clinical devices. Additionally, histological staining and related mRNA expression levels were also analyzed.
RESULTS: The results of skin moisture content, transdermal water loss, and skin elasticity evaluation consistently illustrated that the application of \"CELLADEEP Patch\" led to remarkable skin improvement. And the analysis of histological staining images also confirmed the effectiveness of the \"CELLADEEP Patch\", especially for increasing collagen density. Moreover, the upregulation of Collagen type 1 a (COL1A1) and hyaluronan synthase 3 mRNA expression and the decrease of Matrix metalloproteinase 1 (MMP-1) and Interleukin-1 beta (IL-1β) mRNA expression reflected its wrinkle improvement, moisturizing and anti-inflammation function.
CONCLUSIONS: \"CELLADEPP Patch\", the MTS combined with the
iontophoresis technique, exhibits its effectiveness in moisturizing, skin elasticity improvement, and anti-inflammatory function when applied to ex vivo human-derived skin tissue models in experiments. The study has contributed to the understanding of the \"CELLADEPP Patch\" and laid the foundation for subsequent animal experiments and clinical trials.