PDF(Pubmed)
Abstract：
Iontophoresis enables the non-invasive transdermal delivery of moderately-sized proteins and the needle-free cutaneous delivery of antibodies. However, simple descriptors of protein characteristics cannot accurately predict the feasibility of iontophoretic transport. This study investigated the cathodal and anodal iontophoretic transport of the negatively charged M7D12H nanobody and a series of negatively charged variants with single amino acid substitutions. Surprisingly, M7D12H and its variants were only delivered transdermally by anodal iontophoresis. In contrast, transdermal permeation after cathodal iontophoresis and passive diffusion was
摘要：
离子电渗疗法能够实现中等大小的蛋白质的非侵入性经皮递送和抗体的无针皮肤递送。然而,简单的蛋白质特征描述符不能准确预测离子电渗转运的可行性。这项研究研究了带负电荷的M7D12H纳米抗体和一系列带负电荷的变体的阴极和阳极离子电渗转运，这些变体具有单个氨基酸取代。令人惊讶的是,M7D12H及其变体仅通过阳极离子电渗疗法经皮递送。相比之下,离子电渗疗法和被动扩散后的经皮渗透
