背景技术富血小板血浆(PRP)是通过离心全血制备的自体产物。据报道,PRP具有高的组织修复潜力和抗炎性质。最近,PRP已成为骨关节炎的潜在治疗选择,有助于缓解疼痛和改善机车。然而,PRP的潜在治疗机制和关键生化因素尚不清楚.本研究旨在通过比较使用Luminex测定法从同一患者制备的血清和PRP来评估PRP治疗中涉及的组织修复的主要因素。方法从9名健康志愿者中收集血液样本,制备血清和PRP。PRP是使用DePuySynthesMitek运动医学(Raynham,马萨诸塞州,美国),这是一种市售的PRP制备试剂盒。白细胞计数,血红蛋白水平,在医院的临床实验室中,使用XE-5000™自动血液学系统(Sysmex,神户,日本)。然后使用Luminex测定对血清和PRP进行生物因素的比较分析。结果发现PRP的白细胞和血小板计数明显高于全血,血红蛋白水平较低。此外,PRP含有明显较高水平的各种因素,包括白细胞介素(IL)-1ra,IL-10,IL-13,C-C基序趋化因子配体(CCL)-2,CCL3,CCL4,CCL8,CCL13,CCL21,C-X-C基序趋化因子配体(CXCL)-10,基质金属蛋白酶(MMP)-3,MMP-9,分化簇(CD)40配体,血管内皮生长因子(VEGF),VEGF-C,血小板衍生生长因子(PDGF)-AB,PDGF-BB,和骨形态发生蛋白(BMP)-2。此外,PRP中IL-1ra和IL-4与白细胞计数呈显著相关,而VEGF与血小板计数有显著相关性。结论PRP含有多种因子的含量高于血清。具体来说,抗炎细胞因子IL-1ra的显着增加被认为是PRP的主要治疗机制。
Background Platelet-rich plasma (PRP) is an autologous product prepared by centrifuging whole blood. PRP is reported to have high tissue repair potential and anti-inflammatory properties. Recently, PRP has become a potential treatment option for osteoarthritis, contributing to pain relief and locomotive improvement. However, the underlying therapeutic mechanisms and key biochemical factors in PRP remain unclear. This study aimed to estimate the major factors for tissue repair involved in PRP treatment by comparing between serum and PRP prepared from the same patients using the Luminex assay. Methodology Blood samples were collected from nine healthy volunteers, and serum and PRP were prepared. PRP was prepared using a PEAK©︎ PRP SYSTEM kit of DePuy Synthes Mitek Sports Medicine (Raynham, Massachusetts, USA), which is a commercially available PRP preparation kit. The white blood cell count, hemoglobin level, and platelet count were automatically measured for both whole blood and PRP in the hospital\'s clinical laboratory using the XE-5000™ Automated Hematology System (Sysmex, Kobe, Japan). Comparative analysis of biological factors was then performed using the Luminex assay on serum and PRP. Results PRP was found to have significantly higher white blood cell and platelet counts and lower hemoglobin levels than whole blood. Furthermore, PRP contained significantly higher levels of various factors, including interleukin (IL)-1ra, IL-10, IL-13, C-C motif chemokine ligand (CCL)-2, CCL3, CCL4, CCL8, CCL13, CCL21, C-X-C motif chemokine ligand (CXCL)-10, matrix metalloproteinase (MMP)-3, MMP-9, cluster of differentiation (CD) 40 ligand, vascular endothelial growth factor (VEGF), VEGF-C, platelet-derived growth factor (PDGF)-AB, PDGF-BB, and bone morphogenic protein (BMP)-2. Additionally, IL-1ra and IL-4 showed significant correlations with white blood cell counts in PRP, whereas VEGF had a significant correlation with platelet counts. Conclusions PRP contains various factors in higher quantities than serum. Specifically, the notable increase in the anti-inflammatory cytokine IL-1ra is suggested to play a key role as a major therapeutic mechanism of PRP.