背景:迫切需要更有效,更方便的诊断方法来减轻结核病(TB)的负担。本研究探讨了基于QuantiFERON-TBGoldPlus(QFT-Plus)的多种细胞因子分泌,并筛选具有诊断潜力的最佳细胞因子以区分TB感染状态。
方法:20名活动性肺结核(ATB)患者,15例潜伏性结核感染(LTBI)患者,纳入10例既往结核病患者和10例健康对照(HC).收集全血样品并用QFT-Plus的TB1和TB2抗原刺激。IFN-γ的水平,TNF-α,IL-2、IL-6、IL-5、IL-10、IP-10、IL-1Ra、通过基于Luminex珠的多重测定测量上清液中的CXCL-1和MCP-1。采用受试者工作特征曲线评价细胞因子对不同结核感染状态的诊断准确性。
结果:用QFT-PlusTB1和TB2抗原刺激后,所有细胞因子的水平,除了TB2管中的IL-5,ATB组明显高于HC组。IL-1Ra的水平同时显示出同样最高的AUC,以区分TB感染和HC。其次是TB1管和TB2管中的IP-10水平。此外,IP-10水平显示出区别ATB患者和非ATB患者的最大AUC。同时,在区分ATB患者和LTBI患者的TB1管和TB2管中,IP-10的水平也显示出最大的AUC.
结论:除常规检测IFN-γ外,基于QFT-Plus测量IP-10和IL-1Ra可能具有更巨大的潜力来区分不同的TB感染状态。
BACKGROUND: More efficient and convenient diagnostic method is a desperate need to reduce the burden of tuberculosis (TB). This
study explores the multiple cytokines secretion based on QuantiFERON-TB Gold Plus (QFT-Plus), and screens for optimal cytokines with diagnostic potential to differentiate TB infection status.
METHODS: Twenty active tuberculosis (ATB) patients, fifteen patients with latent TB infection (LTBI), ten patients with previous TB and ten healthy controls (HC) were enrolled. Whole blood samples were collected and stimulated by QFT-Plus TB1 and TB2 antigens. The levels of IFN-γ, TNF-α, IL-2, IL-6, IL-5, IL-10, IP-10, IL-1Ra, CXCL-1 and MCP-1 in supernatant were measured by Luminex bead-based multiplex assays. The receiver operating characteristic curve was used to evaluate the diagnostic accuracy of cytokine for distinguishing different TB infection status.
RESULTS: After stimulation with QFT-Plus TB1 and TB2 antigens, the levels of all cytokines, except IL-5 in TB2 tube, in ATB group were significantly higher than that in HC group. The levels of IL-1Ra concurrently showed the equally highest AUC for distinguishing TB infection from HC, followed by the levels of IP-10 in both TB1 tube and TB2 tube. Moreover, IP-10 levels displayed the largest AUC for distinguishing ATB patients from non-ATB patients. Meanwhile, the levels of IP-10 also demonstrated the largest AUC in both TB1 tube and TB2 tube for distinguishing ATB patients from LTBI.
CONCLUSIONS: In addition to conventional detection of IFN-γ, measuring IP-10 and IL-1Ra based on QFT-Plus may have the more tremendous potential to discriminate different TB infection status.