背景:人类神经系统修复神经细胞的能力有限,这在治疗伤害和疾病方面带来了巨大的挑战。干细胞通过更新自我并发育成几种细胞类型的潜力来识别,使它们成为受损神经元细胞替代的理想候选者。胚胎干细胞的神经元分化在现代医学中具有重要意义。纳米材料在指导该领域的干细胞功能和组织再生方面具有明显的优势。我们试图在这个系统评价中收集数据,分析它们,并报道了纳米材料对胚胎干细胞神经元分化的影响。
方法:国际数据库,如PubMed、Scopus,ISIWebofScience,和EMBASE搜索了有关纳米材料对胚胎干细胞神经元分化影响的可用文章(直到2023年10月)。之后,筛选(按标题,abstract,和全文),选择,并进行了数据提取。此外,根据STROBE检查表进行质量评估。
结果:总计,1507篇文章进行了鉴定和评估,然后只有29篇文章被发现有资格被纳入。九项研究使用了0D纳米材料,十种使用过的一维纳米材料,两种报道的二维纳米材料,八位展示了三维纳米材料的应用。研究中的主要生物材料是基于聚合物的复合材料。三项研究报道了纳米材料对神经分化的负面影响。
结论:神经分化在神经再生医学中至关重要。具有不同特性的纳米材料,特别是那些细胞调节活动和干细胞命运,在神经组织工程方面有很大的潜力。这些发现表明对物理化学线索在神经组织工程中的潜在应用有了新的理解。
BACKGROUND: Humans\' nervous system has a limited ability to repair nerve cells, which poses substantial challenges in treating injuries and diseases. Stem cells are identified by the potential to renew their selves and develop into several cell types, making them ideal candidates for cell replacement in injured neurons. Neuronal differentiation of embryonic stem cells in modern medicine is significant. Nanomaterials have distinct advantages in directing stem cell function and tissue regeneration in this field. We attempted in this systematic review to collect data, analyze them, and report results on the effect of nanomaterials on neuronal differentiation of embryonic stem cells.
METHODS: International databases such as PubMed, Scopus, ISI Web of Science, and EMBASE were searched for available articles on the effect of nanomaterials on neuronal differentiation of embryonic stem cells (up to OCTOBER 2023). After that, screening (by title, abstract, and full text), selection, and data extraction were performed. Also, quality assessment was conducted based on the STROBE checklist.
RESULTS: In total, 1507 articles were identified and assessed, and then only 29 articles were found eligible to be included. Nine studies used 0D nanomaterials, ten used 1D nanomaterials, two reported 2D nanomaterials, and eight demonstrated the application of 3D nanomaterials. The main biomaterial in studies was polymer-based composites. Three studies reported the negative effect of nanomaterials on neural differentiation.
CONCLUSIONS: Neural differentiation is crucial in neurological regenerative medicine. Nanomaterials with different characteristics, particularly those cellular regulating activities and stem cell fate, have much potential in neural tissue engineering. These findings indicate a new understanding of potential applications of physicochemical cues in nerve tissue engineering.