Hippo通路可以调节细胞分裂,分化和凋亡,控制器官的形状和大小。研究组蛋白修饰Hippo通路基因在胚胎干细胞中的分布规律,有助于了解组蛋白修饰和Hippo通路对干细胞自我更新的分子调控机制。在这项研究中,Hippo途径的19个基因,包括YAP,TAZ,选择LATS1/2,MST1和SAV1以及胚胎干细胞中的八种组蛋白修饰来研究组蛋白修饰的空间分布模式。发现组蛋白修饰的分布具有明显的类型特异性和靶区域的位置偏好。H3K4me3和H3K36me3发挥了最重要的调节作用。通过组蛋白修饰的相关性分析,由H3K4ac组成的组蛋白修饰功能簇,在YAP中检测到H3K4me3、H3K9ac和H3K27ac。此外,获得了Hippo途径基因中组蛋白修饰的空间分布模式,为阐明组蛋白修饰调控Hippo通路基因表达的机制提供了新的理论参考,揭示组蛋白修饰通过调控Hippo通路影响胚胎干细胞自我更新的分子调控机制。
Hippo pathway can regulate cell division, differentiation and apoptosis, and control the shape and size of organs. To
study the distribution patterns of histone modifications of Hippo pathway genes in embryonic stem cells is helpful to understand the molecular regulation mechanism of histone modification and Hippo pathway on stem cell self-renewal. In this
study, 19 genes of Hippo pathway including YAP, TAZ, LATS1/2, MST1 and SAV1, and eight histone modifications in embryonic stem cells were chosen to
study the spatial distribution patterns of histone modifications. It was found that there were obvious type specificity and the location preference of target regions in the distributions of histone modifications, and H3K4me3 and H3K36me3 played the most important regulatory roles. Through the correlation analysis of histone modifications, a histone modification functional cluster composed of H3K4ac, H3K4me3, H3K9ac and H3K27ac was detected in YAP. In addition, the spatial distribution patterns of histone modifications in Hippo pathway genes were obtained, which provided a new theoretical reference for elucidating the mechanism of histone modifications regulating the gene expression of Hippo pathway, and for revealing the molecular regulatory mechanism of histone modifications affecting the self-renewal of embryonic stem cells by regulating the Hippo pathway.